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https://www.sciencedirect.com/science/article/pii/S0378517397002445
Stabilization of gene delivery systems by freeze-drying 1. Introduction. Gene therapy is strongly influenced by any progress in the development... 2. Materials and methods. The recombinant adenovirus pAdLuc was generated using a two plasmid system... 3. …Author: Herre Talsma, Jong-Yuh Cherng, Heike Lehrmann, Malgorzata Kursa, Manfred Ogris, Wim E Hennink, Matt ...
https://www.sciencedirect.com/science/article/pii/S0378517303003806
Nov 06, 2003 · Stabilisation by freeze-drying of cationically modified silica nanoparticles for gene delivery We dedicate this paper to Professor Hans E. Junginger on his 60th birthday for his scientific approach, honesty and human warmthAuthor: M. Sameti, G. Bohr, M. N. V. Ravi Kumar, Carsten Kneuer, Udo Bakowsky, Manfred Nacken, Helmut K. Sch...
https://www.ncbi.nlm.nih.gov/pubmed/10361148
Jun 10, 1999 · Freeze-drying and freeze-thawing were used for stabilization of these complexes. The concentration of the sugars is an important factor affecting both the size and transfection capability of the complexes after freeze-drying and freeze-thawing.Author: J.-Y Cherng, P.v.d Wetering, H Talsma, D.J.A Crommelin, W.E Hennink
https://www.deepdyve.com/lp/elsevier/stabilization-of-gene-delivery-systems-by-freeze-drying-b8bxBvIEAi
Freeze-drying of three different forms of gene delivery systems was performed using a controlled two-step drying process and 10% sucrose as lyoprotectant.
https://www.researchgate.net/publication/9051436_Stabilization_by_Freeze-Drying_of_Cationically_Modified_Silica_Nanoparticles_for_Gene_Delivery
Request PDF Stabilization by Freeze-Drying of Cationically Modified Silica Nanoparticles for Gene Delivery Core shell silica particles with a hydrodynamic diameter of 28nm, an IEP of 7.1 and a ...
https://link.springer.com/protocol/10.1007/978-1-62703-140-0_10
Sep 14, 2012 · Lyophilization, also known as freeze-drying, is a widely used method for stabilization, improvement of long-term storage stability, and simplification of the handling of drugs and/or carrier systems. Lyophilization is time- and energy-consuming; hence, optimized processes are required to avoid time loss and higher costs without compromising product stability.Author: Julia Christina Kasper, Sarah Küchler, Wolfgang Friess
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556052/
In solid state, polymer based gene delivery systems have an advantage over lipid based ones because they are less vulnerable to oxidative reactions . Although freeze-drying is a well-established industrial approach, conventional freeze-drying can exacerbate aggregation in its freezing step by concentrating nanosuspension in unfrozen phase.Author: Nasir Mohajel, Abdolhossein R Najafabadi, Kayhan Azadmanesh, Mohsen Amini, Alireza Vatanara, Esmail ...
https://www.intechopen.com/books/recent-advances-in-novel-drug-carrier-systems/gene-delivery-systems-recent-progress-in-viral-and-non-viral-therapy
The use of non-viral gene delivery systems in gene therapy also significantly increased in recent years. Naked plasmid DNA, coated with gold particles was effectively introduced to cells using the non-viral gene gun technique. This technique was first used in plant cells in 1987, and is today commonly used in mammal cells and tissues.Author: Erdal Cevher, Ali Demir Sezer, Emre Şefik Çağlar
http://europepmc.org/articles/PMC3556052
Dec 01, 2011 · It was shown that a freeze drying process with increased cooling rate can improve preservation of biological activity of non-viral gene delivery systems (8), despite the fact that rapid freezing of naked DNA can extensively damage its structure [23,24].
https://rd.springer.com/chapter/10.1007%2F978-1-4939-2383-0_10
Therefore, the development of dehydrated nucleic acid-based formulations represents a valuable approach for stabilization and storage of large standardized batches of naked DNA or lipid/DNA complexes. The most common method taken to dehydrate, and therefore stabilize, macromolecules is freeze-drying.Author: Marion dC. Molina, Nicole M. Payton, Thomas J. Anchordoquy
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