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https://www.sciencedirect.com/science/article/pii/S0378517397002445
Freeze-drying would be an obvious approach for stabilization and storage of large standardized batches. We report here, methods for freeze-drying three different forms of gene delivery reagents assembled into transfection-competent form.Author: Herre Talsma, Jong-Yuh Cherng, Heike Lehrmann, Malgorzata Kursa, Manfred Ogris, Wim E Hennink, Matt ...
https://www.deepdyve.com/lp/elsevier/stabilization-of-gene-delivery-systems-by-freeze-drying-b8bxBvIEAi
Freeze-drying of three different forms of gene delivery systems was performed using a controlled two-step drying process and 10% sucrose as lyoprotectant. Complexes of pCMVL plasmid with transferrin-conjugated polyethylenimine, adenovirus-enhanced transferrinfection consisting of pCMVL/transferrin-polylysine complexes linked to inactivated adenovirus particles, and a recombinant, E1-defective ...
https://www.sciencedirect.com/science/article/pii/S0378517303003806
Nov 06, 2003 · 1. Introduction. Silica nanoparticles have been described as drug delivery systems applied both as excipients in drug delivery systems (Ahola et al., 1999) and as a gel matrix (Bottcher et al., 1999, Kortesuo et al., 1999) with controlled release effects (Kortesuo et al., 2000).Silica nanoparticles are also used as the major component of drug carriers (Shimada et al., 1995) and for in-vitro ...Author: M. Sameti, G. Bohr, M. N. V. Ravi Kumar, Carsten Kneuer, Udo Bakowsky, Manfred Nacken, Helmut K. Sch...
https://www.ncbi.nlm.nih.gov/pubmed/10361148
Jun 10, 1999 · Stabilization of polymer-based gene delivery systems. ... Freeze-drying and freeze-thawing were used for stabilization of these complexes. The concentration of the sugars is an important factor affecting both the size and transfection capability of the complexes after freeze-drying and freeze-thawing. ... Stabilization of polymer-based gene ...Author: J.-Y Cherng, P.v.d Wetering, H Talsma, D.J.A Crommelin, W.E Hennink
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556052/
It was shown that a freeze drying process with increased cooling rate can improve preservation of biological activity of non-viral gene delivery systems (8), despite the fact that rapid freezing of naked DNA can extensively damage its structure [23,24]. Our results also indicated that by applying a similar freeze-drying condition, in N/P ratios ...Author: Nasir Mohajel, Abdolhossein R Najafabadi, Kayhan Azadmanesh, Mohsen Amini, Alireza Vatanara, Esmail ...
https://www.researchgate.net/publication/9051436_Stabilization_by_Freeze-Drying_of_Cationically_Modified_Silica_Nanoparticles_for_Gene_Delivery
Request PDF Stabilization by Freeze-Drying of Cationically Modified Silica Nanoparticles for Gene Delivery Core shell silica particles with a hydrodynamic diameter of 28nm, an IEP of 7.1 and a ...
https://www.researchgate.net/publication/331541398_Lyophilization_of_Synthetic_Gene_Carriers
Lyophilization, also known as freeze-drying, is a widely used method for stabilization, improvement of long-term storage stability, and simplification of the handling of drugs and/or carrier systems.
https://www.deepdyve.com/lp/elsevier/stabilization-of-polymer-based-gene-delivery-systems-ovrv06DK8C
Stabilization of polymer-based gene delivery systems Stabilization of polymer-based gene delivery systems Cherng, J.-Y; Wetering, P.v.d; Talsma, H; Crommelin, D.J.A; Hennink, W.E 1999-06-10 00:00:00 To preserve the size and transfection potential of polymer–plasmid complexes, freeze-drying and freeze-thawing were used for stabilization of these complexes.
https://link.springer.com/protocol/10.1385/1-59259-139-6:225
Strauss G., Schurtenberger P., and Hauser H. (1986) The interaction of saccharides with lipid bilayer vesicles: stabilization during freeze-thawing and freeze-drying. Biochim. Biophys.Author: S. Dean Allison, Thomas J. Anchordoquy
https://link.springer.com/protocol/10.1007/978-1-62703-140-0_10
Sep 14, 2012 · Talsma H, Cherng J-Y, Lehrmann H, Kursa M, Ogris M, Hennink WE, Cotten M, Wagner E (1997) Stabilization of gene delivery systems by freeze-drying. Int J Pharm 157:233–238 CrossRef Google Scholar 2.Author: Julia Christina Kasper, Sarah Küchler, Wolfgang Friess
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