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https://www.hindawi.com/journals/jdd/2017/4070793/
siRNA stabilized for in vivo applications is filtered and reabsorbed in the renal proximal tubule (PT), reducing mRNA expression transiently. Prior siRNA efforts have successfully prevented upregulation of mRNA in response to injury. We proposed reducing constitutive gene and protein expression of LRP2 (megalin) in order to understand its molecular regulation in mice.Author: Michael T. Eadon, Ying-Hua Cheng, Takashi Hato, Eric A. Benson, Joseph Ipe, Kimberly S. Collins, Tho...
https://www.jove.com/video/59201/a-positioning-device-for-placement-mice-during-intranasal-sirna
The head down-and-forward position reduces the possibility of drug leakage from the nose to the lungs while inhaling, favoring direct and selective siRNA delivery to the brain. IN delivery using the mouse positioning device does not require …Author: Irfan Ullah, Irfan Ullah, Kunho Chung, Jagadish Beloor, Sang-Kyung Lee, Priti Kumar
https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rnai-sirna/tech-notes/performing-rnai-experiments-in-animals.html
More recently, low volume, normal pressure intravenous delivery of a modified siRNA targeting apolipoprotein B in mice resulted in gene silencing in the liver and jejunum. The siRNA was conjugated with cholesterol to provide targeted delivery, and included backbone and sugar modifications to enhance serum stability [6].
https://3ejrvdou7mi16guil1ho2me1-wpengine.netdna-ssl.com/wp-content/uploads/sites/21/2019/02/InVivoTransfection3.pdf
Delivery of sirNA to the mouse brain by systemic injection of targeted exosomes Lydia Alvarez-Erviti1,2, Yiqi Seow 1,2, HaiFang Yin , Corinne Betts , Samira Lakhal 1 & Matthew J A Wood 1Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2943428/
Aug 22, 2008 · Mouse experiments with scFvCD7/siRNA delivery. Human cell engrafted mice were iv-injected with scFvCD7-9R/siRNA complexes at a 5:1 molar ratio at a dose of 50 μg siRNA per injection in 5% glucose in a volume of 200 μl. In infection experiments, Hu-PBL mice were ip injected on day 16 post-transplantation with 10000 TCID 50 of HIV BaL in a 100 ...Author: Priti Kumar, Hong Seok Ban, Sang Soo Kim, Haoquan Wu, Todd Pearson, Dale L. Greiner, Amale Laouar, J...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204174/
Aug 11, 2014 · We have used α-Syn small interfering RNA (siRNA) to reduce total and aggregated α-Syn levels in mouse brains. To achieve widespread delivery of siRNAs to the brain we have peripherally injected modified exosomes expressing Ravies virus glycoprotein loaded with siRNA. Normal mice were analyzed 3 or 7 days after injection.Author: JM Cooper, PB Wiklander, JZ Nordin, R Al-Shawi, MJ Wood, M Vithlani, AH Schapira, JP Simons, S El-An...
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