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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2692493/
Jun 01, 2009 · Local delivery of siRNA to the CNS is a feasible option for targeting CNS-based diseases, though systemic delivery strategies may be clinically more attractive. The delivery of siRNA targeting the NR2B subtype of the NMDA receptor using intrathecal injection led to a 83% decrease in the protein’s expression level in the rat brain 70 .Author: Kun Gao, Leaf Huang
https://www.springer.com/gp/book/9781493931118
This volume provides a collection of cutting-edge strategies in siRNA delivery that were developed and refined over the years with tried-and-true methods. Written by a team of internationally renowned authors, this book describes, in detail, a variety of successful siRNA delivery methods, including
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2977003/
Jun 11, 2010 · The site of action of siRNA therapeutics is the cytosol. The barriers to siRNA delivery are multiple and depend on the targeted organs and the administration routes. In general, locoregional delivery of siRNA has fewer barriers compared to systemic delivery.Author: Jie Wang, Ze Lu, M. Guillaume Wientjes, Jessie L.-S. Au
https://link.springer.com/book/10.1007/978-1-4939-3112-5
Comprehensive and authoritative, SiRNA Delivery Methods: Methods and Protocols, will provide researchers, educators, clinicians, and biotechnology specialists with a broad understanding of the issues in siRNA delivery and how they can be overcome strategically.
https://www.sciencedirect.com/science/article/pii/S0168365919307230
Materials and methods ... In vivo distribution of siRNA delivery systems after intravenous injection. (A) CBSA/FAM-siRNA, CBSA/[email protected], and CBSA/[email protected] (green) biodistribution in the lung at 4 h, 12 h and 24 h post-injection imaged by laser confocal microscopy. DAPI was used to …Author: Liuwan Zhao, Chunyan Gu, Ye Gan, Lanlan Shao, Hongwei Chen, Hongyan Zhu
https://www.sciencedirect.com/science/article/pii/S1818087614000646
If siRNA delivery provokes unacceptable toxicity on either a cellular or systemic level, even the most efficacious siRNA delivery system will be rendered useless. Viral vectors, which were among the first vehicles to be studied for siRNA delivery, can induce unacceptable levels of toxicity through the activation of immune responses .Author: Cong-fei Xu, Jun Wang
https://www.labome.com/method/siRNAs-and-shRNAs-Tools-for-Protein-Knockdown-by-Gene-Silencing.html
Additionally, delivery of the siRNA or shRNA may not be possible in certain cell types. Some cells do not tolerate transfection and they may not be susceptible for delivery by viral vectors. An important factor in choosing between siRNA- and shRNA-mediated gene silencing methods is the length of both the assay and the half-life of the target ...Author: Erin P O’Keefe
https://www.amazon.com/SiRNA-Delivery-Methods-Protocols-Molecular/dp/1493949101
Comprehensive and authoritative, SiRNA Delivery Methods: Methods and Protocols, will provide researchers, educators, clinicians, and biotechnology specialists with a broad understanding of the issues in siRNA delivery and how they can be overcome strategically.Author: Kato Shum
http://altogenlabs.com/rna-interference-rnai-services/in-vivo-sirna-delivery-and-tissue-targeting/
In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) In Vivo siRNA Delivery and Tissue Targeting. RNAi-induced gene silencing is used to study gene function in cultured cells (in vitro).Most often, these effects are induced by the introduction of small-interfering RNA (siRNA) or microRNA (miRNA).
https://www.thermofisher.com/us/en/home/life-science/rnai/delivering-rnai-to-cells-transfection-and-viral-delivery.html.html
Viral delivery can also be used to create stable cell lines with inducible RNAi expression or to express RNAi sequences with tissue-specific promoters. Importance of minimizing transfection-mediated cytotoxicity. The delivery of RNAi reagents, or the delivery method itself, can give rise to cytotoxicity in gene silencing experiments.
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