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https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies.html
Viral delivery: Highest efficiency amongst gene delivery methods (80–90% transduction efficiency in primary cells) Works well with difficult to transfect cell types; Can be used for in vivo delivery of nucleic acids; Can be used for making stable cell lines (retroviral …
https://www.sciencedirect.com/science/article/pii/S0169409X98000684
2. Gene delivery by electroporation. In vivo electrically mediated gene transfer has been performed only in animal models to date. The methods and animal models for each published study differ, and the majority of the experimental work has utilized reporter genes to investigate and develop delivery methods.Author: Mark J Jaroszeski, Richard Gilbert, Claude Nicolau, Claude Nicolau, Richard Heller
https://www.btxonline.com/applications/in-vivo-electroporation/invasive-electrodes.html
Versatile electroporation system suitable for applications for gene, drug and protein delivery including: mammalian cells, in vivo, ex vivo, in ovo tissue, ex plant, nuclear transfer and CRISPR NEW! Now available with Pulse Switcher for automatic polarity switching!
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012686/
Gene therapy may represent a promising alternative strategy for cardiac muscle regeneration. In vivo electroporation, a physical method of gene transfer, has recently evolved as an efficient method for gene transfer. In the current study, we investigated the efficiency and safety of a protocol involving in vivo electroporation for gene transfer to the beating heart.Author: Erick L. Ayuni, Amiq Gazdhar, Marie Noelle Giraud, Alexander Kadner, Mathias Gugger, Marco Cecchini,...
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0014467
Gene therapy may represent a promising alternative strategy for cardiac muscle regeneration. In vivo electroporation, a physical method of gene transfer, has recently evolved as an efficient method for gene transfer. In the current study, we investigated the efficiency and safety of a protocol involving in vivo electroporation for gene transfer to the beating heart. Adult male rats were ...Author: Erick L. Ayuni, Amiq Gazdhar, Marie Noelle Giraud, Alexander Kadner, Mathias Gugger, Marco Cecchini,...
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/electroporation
Almost any tissue can be targeted with electroporation, including muscle, skin, heart, liver, lung, and vasculature. This chapter will provide an overview of the theory of electroporation for the delivery of DNA both in individual cells and in tissues and its application for in …
https://www.intechopen.com/books/application-of-nanotechnology-in-drug-delivery/electroporation-advantages-and-drawbacks-for-delivery-of-drug-gene-and-vaccine
Electroporation was introduced in the 1960s and comprises the application of controlled electric fields to facilitate cell permeabilization. The success of in vitro delivery by electroporation has led to the development of in vivo applications [Takei et al., 2008].The first in vitro and in vivo attempts to use electroporation in gene transfer were demonstrated in 1982 and 1991, respectively ...Author: Azam Bolhassani, Afshin Khavari, Zahra Orafa
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC534104/
Direct visualization of electroporation-assisted in vivo gene delivery to tumors using intravital microscopy – spatial and time dependent distribution. Maja Cemazar, 1 Ian Wilson, 2 Gabi U Dachs, 2, 3 Gillian M Tozer, 2, 4 and Gregor Sersa 1Author: Maja Cemazar, Ian Wilson, Gabi U Dachs, Gillian M Tozer, Gregor Sersa
https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(00)90124-2
In the case of in vivo gene delivery by electroporation, four critical steps in the transport process have been identified. The first step involves either association, binding, or proximity between cell membranes and nucleic acids. Virtually all studies that examine temporal relationships between poration and nucleic acid administration have ...
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