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https://www.sciencedirect.com/science/article/pii/S0169409X16302368
For siRNA delivery, an 8-arm peptide, H 3 K8b, was complexed with siRNA targeting β-galactosidase and applied to mouse endothelial cells that were stably expressing β-galactosidase. The branched peptide was capable of reducing β-galactosidase expression by 80% at a siRNA concentration of 300 nM.Author: Wanyi Tai, Xiaohu Gao
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425738/
Apr 10, 2014 · Delivery of siRNA into keratinocytes using SPACE peptide The ability of SPACE-peptide to facilitate internalization of GAPDH siRNA was verified ( Fig. 7 ). GAPDH siRNA conjugated to SPACE peptide and co-incubated with 10 mg/mL free SPACE peptide resulted in 83.3±3.0% knockdown relative to …Author: Ming Chen, Michael Zakrewsky, Vivek Gupta, Aaron C. Anselmo, Deborah H. Slee, John A. Muraski, Samir...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3179050/
Sep 20, 2011 · The ability of SPACE peptide to deliver siRNA was tested in vivo using two targets, interleukin-10 and GAPDH. Conjugation of the peptide to siRNA led to their enhanced absorption into skin and knockdown of corresponding protein targets.Author: Tracy Hsu, Samir Mitragotri
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5410048/
Apr 28, 2017 · Secondary structure was determined when peptide is free in solution, mixed with siRNA at a peptide:siRNA molar ratio of R = 20, or when liposomes are added to the peptide:siRNA mix at a lipid/peptide ratio of r = 5 (Fig. 1 a, b).Author: Anaïs Vaissière, Gudrun Aldrian, Karidia Konate, Mattias F. Lindberg, Carole Jourdan, Anthony Telmar...
https://www.researchgate.net/publication/38039955_Peptide_Mediated_siRNA_Delivery
Here we describe a peptide/siRNA quaternary complex that functions as an siRNA delivery system. The rational design of a peptide assembly is inspired by the viral capsids, but not derived from them.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765502/
These promising preliminary studies clearly illustrate the efficacy of peptide-targeted delivery of siRNA cargos via nanogel carriers. The lack of toxicity observed is of particular interest, given the high toxicity observed for some cationic lipid-based siRNA targeting methods, which limits the maximum doses that can be delivered, and also compromises the potential for in vivo delivery.Author: William H. Blackburn, Erin B. Dickerson, Michael H. Smith, John F. McDonald, L. Andrew Lyon
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