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https://www.pnas.org/content/115/26/E5859
Jun 26, 2018 · Of great significance for further advances in vaccinology and immunology with the advent of advanced cancer immunotherapies using chimeric antigen receptor T cells is the delivery of oligonucleotides, including mRNA, into lymphocytes (18⇓–20).Author: Colin J. McKinlay, Nancy L. Benner, Ole A. Haabeth, Robert M. Waymouth, Paul A. Wender
https://www.nature.com/articles/s41467-018-04791-8
Jun 15, 2018 · RNA delivery for disease treatment often has low uptake efficiencies and cytotoxicity. Here the authors produce extracellular vesicles from red blood cells for in vivo cargo delivery.Author: Waqas Muhammad Usman, Tin Chanh Pham, Yuk Yan Kwok, Luyen Tien Vu, Victor Ma, Boya Peng, Yuen San Ch...
https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rnai-sirna/tech-notes/rnai-how-to--for-new-users.html
Delivery into primary cells and suspension cells, however, can be problematic, if not impossible, using standard transfection methodologies. In these cases, electroporation using a specialized, gentle-on-cells buffer and optimized pulsing conditions generally results in very efficient siRNA delivery without compromising cell viability.
https://www.sciencedaily.com/releases/2017/09/170920155014.htm
Sep 20, 2017 · By delivering strands of genetic material known as messenger RNA (mRNA) into cells, researchers can induce the cells to produce any protein encoded by the mRNA. This technique holds great potential for administering vaccines or treating diseases such as cancer,...
http://news.mit.edu/2017/bio-inspired-approach-rna-delivery-0920
Sep 19, 2017 · By delivering strands of genetic material known as messenger RNA (mRNA) into cells, researchers can induce the cells to produce any protein encoded by the mRNA. This technique holds great potential for administering vaccines or treating diseases such as cancer, but achieving efficient delivery...
https://www.nature.com/articles/nature18300
The systemic delivery of vaccine antigens into the dendritic or antigen-presenting cells of the immune system faces many technical challenges. This study reports the development of a nanoparticle RNA vaccine that preferentially targets dendritic cells after systemic administration.Author: Lena M. Kranz, Mustafa Diken, Heinrich Haas, Sebastian Kreiter, Carmen Loquai, Kerstin C. Reuter, Ma...
https://genomemedicine.biomedcentral.com/articles/10.1186/s13073-017-0450-0
Jun 27, 2017 · Nanoparticle encapsulation of RNA physically protects nucleic acids from degradation and, depending on the specific chemistry, can aid in cellular uptake and endosomal escape. Given their high degree of chemical flexibility, polymers are commonly used materials for nanoparticle-based delivery …Author: James C. Kaczmarek, Piotr S. Kowalski, Daniel G. Anderson
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2977003/
Jun 11, 2010 · RNA interference (RNAi) is a highly efficient regulatory process that causes posttranscriptional gene silencing in most eukaryotic cells (1). RNAi represents a promising new approach for producing gene-specific inhibition and knockouts, producing transgenic animal models, and designing new therapeutics.Author: Jie Wang, Ze Lu, M. Guillaume Wientjes, Jessie L.-S. Au
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342945/
Jan 01, 2012 · One very promising approach is the application of cell-specific aptamers as delivery vehicles for drug molecules into target cells or tissues. One auspicious example is the successful selection of an RNA aptamer binding to PSMA (prostate-specific membrane antigen) presented on the surface of prostate cancer cells. 17 Upon binding, anti-PSMA aptamers undergo receptor-mediated …Author: Cindy Meyer, Katja Eydeler, Eileen Magbanua, Tijana Zivkovic, Nicolas Piganeau, Inken Lorenzen, Joac...
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