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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753151/
Vighi et al29 also used cationic SLNs as a gene vector and the protamine was used as a transfection promoter. It was found that the lower the protamine amount on the surface of the SLN, the lower the efficiency of transfection. In this research, an SLN/protamine/DNA gene delivery system was designed to improve transfection efficiency.Author: Sai-Nan He, Yun-Long Li, Jing-Jing Yan, Wei Zhang, Yong-Zhong Du, He-Yong Yu, Fu-Qiang Hu, Hong Yuan
https://www.sciencedirect.com/science/article/pii/S0378517310001997
Our results indicated that lipid nanoparticles with 20 wt%OA/protamine/DNA was an effective non-viral gene transfer vector even in the presence of serum, and its gene expression was durable as long as 72 h, which may be optimal for future in vivo application of this gene delivery system.Author: Hong Yuan, Wei Zhang, Yong-Zhong Du, Fu-Qiang Hu
https://www.nature.com/articles/3301840
Jan 13, 2003 · The combination of targeting and protamine condensation obviated the need for partial hepatectomy, commonly required to obtain efficient gene delivery in …Author: M A Arangoa, N Düzgüneş, C Tros de Ilarduya
https://www.ncbi.nlm.nih.gov/pubmed/12525832
The combination of targeting and protamine condensation obviated the need for partial hepatectomy, commonly required to obtain efficient gene delivery in this organ. Since protamine sulfate has been proven to be non-toxic in humans, the novel liver-specific vector described here may be useful for the delivery of clinically important genes to ...Author: M A Arangoa, N Düzgüneş, C Tros de Ilarduya
https://journals.sagepub.com/doi/abs/10.1177/0883911506070816
Nov 01, 2006 · Protamine is an FDA-approved compound with a documented safety profile that facilitates efficient plasmid condensation for gene delivery by various types of …Author: Yuri Tsuchiya, Tsuyoshi Ishii, Yoshio Okahata, Toshinori Sato
https://www.sciencedirect.com/science/article/pii/S0076687903730213
Liposome–protamine–DNA complex (LPD), as a novel nonviral vector, was developed on the basis of our understanding about the cellular and molecular barriers to cationic lipid–based gene delivery systems.1, 2 Although cationic liposome–DNA complexes (“lipoplexes”) have shown promise in gene transfection both in vitro and in vivo ...Author: Jing Shi Zhang, Song Li, Leaf Huang
https://aip.scitation.org/doi/10.1063/1.4790588
Understanding the internal packing of gene carriers is a key-factor to realize both gene protection during transport and de-complexation at the delivery site. Here, we investigate the structure of complexes formed by DNA fragments and protamine, applied in gene delivery. We found that complexes are charge- and size-tunable aggregates, depending on the protamine/DNA ratio, hundred nanometers in ...Author: Simona Motta, Paola Brocca, Elena Del Favero, Valeria Rondelli, Laura Cantù, Augusto Amici, Daniela ...
https://www.nature.com/articles/7701121
Feb 08, 2008 · An efficient liposomal gene delivery vehicle using Sendai F/HN proteins and protamine ... An efficient liposomal gene delivery vehicle using Sendai …Author: H S Kim, J S Kim, Y K Lee, K H Koo, Y S Park
https://www.cell.com/molecular-therapy-family/molecular-therapy/pdf/S1525-0016(16)41018-X.pdf
[Effect on the gene expression] Gal-PEG-C added to the plasmid / protamine complex prior to the incubation with the cells extremely enhanced the gene-expression, and 54-fold higher expression of luciferase than that without sugar-PEG-C was observed by the plasmid / protamine / Gal-PEG-C complex prepared at 1 : 1.5 : 8 in weight.
https://journals.sagepub.com/doi/10.1177/0883911506070816
It also improves adenoviral vector-mediated gene transfer as a transfection accelerator. However, there is no consensus as to the mechanism of protamine on gene delivery into cells. To analyze the uptake and subcellular distribution, plasmid and protamine were labeled with FITC and Texas-Red, respectively.Author: Yuri Tsuchiya, Tsuyoshi Ishii, Yoshio Okahata, Toshinori Sato
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