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https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies.html
Gene delivery technologies Transfection technologies available today can be broadly classified into three groups: chemical, biological, and physical. No one method can be applied to all cells and all experiments.
https://link.springer.com/chapter/10.1007%2F978-3-319-53457-2_6
May 24, 2017 · However, the immunogenic and mutagenic side effects of viral vector as well as toxicity and low efficacy of nonviral carriers are limiting their application. In this respect, naked NAs delivery by physical methods could be the safest procedure for gene therapy strategies if …Author: María José Herrero, Luis Sendra, Antonio Miguel, Salvador F. Aliño, Salvador F. Aliño
https://www.sciencedirect.com/science/article/pii/B9780123849649000037
Gene delivery using nonviral methods is an exciting research area that has been explored for intracellular transgene expression in gene therapy. Nonviral gene delivery is achieved using both physical and chemical methods for safe and efficient transgene expression in target cells.Author: Kaustubh A. Jinturkar, Mohan N. Rathi, Ambikanandan Misra
https://www.ncbi.nlm.nih.gov/pubmed/23099792
Ultimately, electroporation exhibits a high transfection efficiency in many cell types, which is highly desirable for tissue engineering applications, but electroporation and other physical non-viral gene delivery methods are still limited by poor cell viability.Author: Adam Joseph Mellott, M. Laird Forrest, Michael S. Detamore
https://www.researchgate.net/publication/279871090_Gene_Delivery_Using_Physical_Methods
This chapter focuses on the delivery of DNA by various physical methods such as electroporation (EP), sonoporation, microinjection, particle bombardment (gene gun), and hydrodynamic injection with ...
http://www.biologydiscussion.com/gene/gene-transfer/physical-methods-of-gene-transfer-genetics/72013
ADVERTISEMENTS: The following points highlight the ten main physical methods of gene transfer. The methods are: 1. Biolystic or Particle Bombardment 2. Electroporation 3. Microinjection 4. Pollen Transformation 5. Liposome Mediated Transfer 6. Microlaser 7. Macro-Injection 8. Silicon Carbide Fiber (SCF) Mediated Transfer 9. Poly Ethylene Glycol (PEG) Mediated Transformation 10. Ultrasound ...
https://www.slideshare.net/Aparajitha_Anne/gene-delivery-system
May 19, 2012 · Conclusion:• Gene therapy utilizes the delivery of DNA into cells, which can be done by several ways by a number of methods that is some of them are by viral methods and some are by the non-viral methods few are by the Hybrid methods.•
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417261/
Mar 12, 2019 · The viral gene delivery systems have discussed for the viral vectors based on DNA, RNA and oncolytic viral vectors. The non-viral gene delivery systems have also treated for the physicochemical approaches such as physical methods and chemical methods.Author: YK Sung, SW Kim
https://www.sciencedirect.com/science/article/pii/S0065266014000029
Apart from vector-based systems, physical methods of gene delivery are unique in eliminating the need for a special carrier to transfer DNA into cells. The physical methods employ physical forces to create transient pores in plasma membrane so that “naked” DNA molecules can pass through.Author: Mohammad Alsaggar, Dexi Liu
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