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https://link.springer.com/chapter/10.1007%2F978-3-319-53457-2_6
May 24, 2017 · Physical Methods of Gene Delivery. Abstract. Gene therapy can be defined as the use of nucleic acids (NAs) as medicines with the aim of correcting a deficient gene expression, introducing new functions in the cell, repairing mutations and modulating the gene expression.Author: María José Herrero, Luis Sendra, Antonio Miguel, Salvador F. Aliño, Salvador F. Aliño
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5102682/
Physical gene delivery methods attempt to deliver nucleic acids directly to the cell, and attempt to avoid complications associated with targeting, endocytotic pathways and immunogenicity. 89 However, physical gene delivery has its own set of advantages and disadvantages, which limits its use for certain applications. Microinjection is a technique that directly delivers DNA to the cell nucleus, whereas …Author: Adam Joseph Mellott, M. Laird Forrest, Michael S. Detamore
https://www.researchgate.net/publication/318020480_Physical_Methods_of_Gene_Delivery
Due to the advancement of physical technology and nanotechnology, advanced physical gene delivery methods such as electroporation, magnetoporation, sonoporation and optoporation have been extensively developed and are receiving increasing attention, which have …
https://www.sciencedirect.com/science/article/pii/B9780123849649000037
The various physical methods used for gene delivery are naked DNA injection, electroporation, hydrodynamic gene therapy, particle bombardment with a gene gun, microinjection, sonoporation/ultrasound, iontophoresis, magnetofection, laser beam gene transduction, and impalefection.Author: Kaustubh A. Jinturkar, Mohan N. Rathi, Ambikanandan Misra
https://www.mdpi.com/journal/pharmaceutics/special_issues/genedelivery
Despite such potential, therapeutic success still largely depends on the development of safe and effective ways to deliver nucleic acids within targeted cells. Gene delivery technologies are therefore in the limelight. They are broadly classified into two groups: vector-based and physical methods.
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