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http://www.biologydiscussion.com/gene/gene-transfer/physical-methods-of-gene-transfer-genetics/72013
The following points highlight the ten main physical methods of gene transfer. The methods are: 1. Biolystic or Particle Bombardment 2. Electroporation 3. Microinjection 4. Pollen Transformation 5. Liposome Mediated Transfer 6. Microlaser 7. Macro-Injection 8. …
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies.html
Gene delivery technologies Transfection technologies available today can be broadly classified into three groups: chemical, biological, and physical. No one method can be applied to all cells and all experiments.
https://www.sciencedirect.com/science/article/pii/S0065266014000029
The rationale of physical methods for gene delivery at cellular level is to overcome membrane barrier and facilitate gene transfer into cells by generating transient pores or defects in plasma membrane through which DNA can get into cells.Author: Mohammad Alsaggar, Dexi Liu
https://www.ncbi.nlm.nih.gov/pubmed/23099792
However, there is no single non-viral gene delivery strategy that "fits-all" cell types and tissues. Thus, there is a compelling opportunity to examine different non-viral vectors, especially physical vectors, and compare their relative degrees of success.Author: Adam Joseph Mellott, M. Laird Forrest, Michael S. Detamore
https://www.sciencedirect.com/science/article/pii/B9780123849649000037
Gene delivery via LBTS is not extensively used due to the high cost and the physical size of the laser sources required. In the 1980s, various studies were carried out for in vitro gene delivery using this method , , and since then, many studies have been published , , , . Most recently, Zeira et al. revealed that gene delivery in muscle by the ...Author: Kaustubh A. Jinturkar, Mohan N. Rathi, Ambikanandan Misra
https://www.researchgate.net/publication/279871090_Gene_Delivery_Using_Physical_Methods
This chapter focuses on the delivery of DNA by various physical methods such as electroporation (EP), sonoporation, microinjection, particle bombardment (gene gun), and hydrodynamic injection with ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722627/
Although gene therapy as a treatment for disease holds great promise, progress in developing effective clinical protocols has been slow. The problem lies in the development of safe and efficient gene-delivery systems. This review will evaluate the problems and the potential solutions in this new field of medicine.
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies/other-physical-delivery-methods.html
Physical gene delivery methods other than electroporation include biolistic particle delivery, direct microinjection, and laser-mediated transfection.Although these physical methods differ in the tools they employ, they all enable the direct transfer of nucleic acids into the cytoplasm or the nucleus by membrane penetration without using chemicals or viruses.
https://en.wikipedia.org/wiki/Electroporation
Electroporation mediated delivery of a plasmid coding gene for interleukin-12 (pIL-12) was performed and safety, tolerability and therapeutic effect were monitored. Study concluded, that gene electrotransfer with pIL-12 is safe and well tolerated.
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