We have collected information about Lentiviral Delivery Of Rnai In Hippocampal Neurons for you. Follow the links to find out details on Lentiviral Delivery Of Rnai In Hippocampal Neurons.
https://www.sciencedirect.com/science/article/pii/S0076687906060460
Lentiviral Transduction of Dissociated Hippocampal Neurons. Hippocampal neurons are typically prepared from rat fetuses at embryonic day 18–19 (E18‐E19) or from mouse fetuses at a comparable developmental stage. A number of protocols have been developed to isolate and culture hippocampal neurons (Banker et al., 1998). In the protocol we ...Author: Justyna Janas, Jacek Skowronski, Linda Van Aelst
https://www.researchgate.net/publication/7303275_Lentiviral_Delivery_of_RNAi_in_Hippocampal_Neurons
Lentiviral delivery of shRNA is an emerging approach to perform loss-of-function experiments in nondividing cells such as hippocampal neurons (Janas et al., 2006). We thus generated FIV vectors ...
https://www.ncbi.nlm.nih.gov/pubmed/16472690
Effective delivery of siRNA molecules into target cells or tissues is critical for successful RNA interference (RNAi) application. Here, we describe the use of human immunodeficiency virus type 1 (HIV-1)-based lentiviral vectors for delivery of short hairpin RNA (shRNA), a precursor of siRNA, into primary neurons to suppress gene expression.Author: Justyna Janas, Jacek Skowronski, Linda Van Aelst
https://mniopenresearch.org/articles/1-2
Dec 05, 2017 · A major advantage of lentiviral particles is that they can be used in post-mitotic cells and protocols for the lentiviral-delivery of shRNAs into neurons have been described (Harper & Gonzalez-Alegre, 2008). Lentivirus has also been used for the delivery of …Author: Brigitte Ritter, Brigitte Ritter, Shawn M. Ferguson, Pietro De Camilli, Peter S. McPherson
https://www.researchgate.net/publication/236916107_Lentiviral_Vector_Production_Titration_and_Transduction_of_Primary_Neurons
Lentiviral Vector Production, Titration, and Transduction of Primary Neurons Article (PDF Available) in Methods in molecular biology (Clifton, N.J.) 1018:119-31 · May 2013 with 1,334 Reads
https://bmcneurosci.biomedcentral.com/articles/10.1186/1471-2202-5-4
Jan 30, 2004 · However the expression of Cre and GFP was to some extent cell type specific. For example the neurons in the dentate gyrus were more frequently transduced than most other neurons in the hippocampal formation, whereas neurons in most of the CA3 region (except the terminal part) were only very rarely transduced.Author: Bushra Y Ahmed, Sridhara Chakravarthy, Ruben Eggers, Wim T J M C Hermens, Jing Ying Zhang, Simone P ...
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The pLenti6/BLOCK-iT™-DEST expression vector provided in the BLOCK-iT™ Lentiviral RNAi Expression System can be used to efficiently introduce and stably express short hairpin RNA (shRNA) in vivo from a lentiviral vector.A novel cloning process places an ~50-bp DNA oligonucleotide immediately following a U6 pol III promoter into the BLOCK-iT™ U6 entry vector.
https://www.liebertpub.com/doi/10.1089/104303403322611809
Jul 07, 2004 · RNA interference (RNAi) is an evolutionarily conserved mechanism of posttranscriptional gene-specific silencing. For in vivo applications, RNAi has been hampered until recently by inefficient delivery methods and by the transient nature of the gene suppression. Lentiviral vectors (LVs) hold great promise for gene therapeutic applications ...Author: Chris Van Den Haute, Kristel Eggermont, Bart Nuttin, Zeger Debyser, Veerle Baekelandt
https://bmcneurosci.biomedcentral.com/articles/10.1186/1471-2202-10-2
Jan 13, 2009 · Therefore, amongst hippocampal granule cells, only adult-generated neurons are target for lentivirus-mediated transgene delivery. These properties make lentiviral vectors excellent systems for overexpression or knockdown of genes in neuronal progenitor cells, immature neurons and adult-generated neurons of the mouse hippocampus in vivo.Author: Lenneke Wa van Hooijdonk, Muhammad Ichwan, Thomas F Dijkmans, Theo G Schouten, Marijke Wa de Backer,...
https://www.nature.com/articles/npp2013220
Aug 28, 2013 · Lentiviral Delivery of a Vesicular Glutamate Transporter 1 (VGLUT1)-Targeting Short Hairpin RNA Vector Into the Mouse Hippocampus Impairs Cognition ... and in …Author: Madeleine V. King, Nisha Kurian, Si Qin, Nektaria Papadopoulou, Ben H. C. Westerink, Thomas I. Creme...
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