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http://www.jbc.org/content/276/37/35103.long
Sep 14, 2001 · We have developed a lipid-mediated delivery system that can deliver functional proteins or other bioactive molecules into living cells. This delivery system is composed of a new trifluoroacetylated lipopolyamine (TFA-DODAPL) and dioleoyl phosphatidylethanolamine (DOPE).Author: Olivier Zelphati
https://www.researchgate.net/publication/11894701_Intracellular_Delivery_of_Proteins_with_a_New_Lipid-mediated_Delivery_System
Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System Article (PDF Available) in Journal of Biological Chemistry 276(37):35103-10 · October 2001 with 67 Reads
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151968/
Oct 16, 2007 · Protein delivery represents a powerful tool for experiments in live cells including studies of protein-protein interactions, protein interference with blocking antibodies, intracellular trafficking and protein or peptide biological functions. Most available reagents dedicated to the protein delivery allow efficient crossing of the plasma membrane.Author: Claire O. Weill, Stéphanie Biri, Abdennaji Adib, Patrick Erbacher
https://www.nature.com/articles/nbt.3081
Oct 30, 2014 · Efficient protein delivery using cationic lipid transfection reagents enables high efficiency protein-based genome editing in vivo and in vitro. Efficient intracellular delivery of proteins is ...Author: John A. Zuris, John A. Zuris, David B. Thompson, David B. Thompson, Yilai Shu, John P. Guilinger, Jo...
https://www.cell.com/cell/fulltext/S0092-8674(15)00315-3
Apr 23, 2015 · Here, we describe a method for the efficient delivery of native proteins and other macromolecules, such as small RNAs, into primary cells. We discovered that a combination of small molecules drives the highly efficient intracellular delivery of native proteins, independent of any transduction peptide.Author: Diego S. D’Astolfo, Romina J. Pagliero, Anita Pras, Wouter R. Karthaus, Hans Clevers, Vikram Prasad,...
https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(16)30029-4
Jan 01, 2015 · The intracellular delivery of biologically active protein represents an important emerging strategy for both fundamental and therapeutic applications. Here, we optimized in vitro delivery of two functional proteins, the β-galactosidase (β-gal) enzyme and the anti-cytokeratin8 (K8) antibody, using liposome-based formulation.Author: Benoît Chatin, Benoît Chatin, Mathieu Mével, Mathieu Mével, Julie Devallière, Julie Devallière, Laur...
https://www.onlinelibrary.wiley.com/doi/abs/10.1002/anie.201311245
A study of the structure–function relationship reveals that the electrostatic and hydrophobic interactions between the lipidoids and the protein play a vital role in the formation of protein–lipidoid nanocomplexes and intracellular delivery.Author: Ming Wang, Kyle Alberti, Shuo Sun, Carlos Luis Arellano, Qiaobing Xu
https://www.sciencedirect.com/science/article/pii/S016836590500026X
Apr 18, 2005 · To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins or other bioactive macromolecules into a specific cell, a di-block copolymer conjugate, poly(l-lysine)–poly(ethylene glycol)–folate (PLL–PEG–FOL), was synthesized.The PLL–PEG–FOL conjugate was physically complexed with fluorescein isothiocyanate conjugated bovine serum albumin (FITC ...Author: Sun Hwa Kim, Ji Hoon Jeong, Cheol O. Joe, Tae Gwan Park
https://biotechtuesday.com/content/lipid%E2%80%90mediated-formulation-protein-delivery
We have IP for both chemical modification of proteins and lipid-based delivery materials and the intracellular delivery nanoparticle formulation. Based on this portfolio, we are capable of developing new products through research, development and marketing, in particular focusing on cancer therapy.
https://pubs.acs.org/doi/10.1021/jacs.8b12705
Thus far, direct intracellular delivery of proteins has remained challenging. We developed a method termed iTOP, for induced transduction by osmocytosis and propanebetaine, in which a combination of NaCl hypertonicity-induced macropinocytosis and a transduction compd. (propanebetaine) induces the highly efficient transduction of proteins into a ...Author: Shunzhi Wang, Yijing Chen, Shuya Wang, Peng Li, Chad A. Mirkin, Omar K. Farha
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