We have collected information about Hydrodynamic Plasmid Delivery for you. Follow the links to find out details on Hydrodynamic Plasmid Delivery.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064722/
Hydrodynamic delivery is one of non-viral methods developed originally for intracellular gene delivery but later found applicable to delivery of other macromolecules such as oligo nucleotides, RNA, proteins, and the compounds that are not permeable to cell membrane.Author: Barbara Bonamassa, Li Hai, Dexi Liu
https://www.sciencedirect.com/science/article/pii/S1525001616327198
Hydrodynamic gene delivery has been shown to be able to promote both humoral- and cell-based immunity in animals in a gene dependent manner. Hydrodynamic gene delivery generates much more gene product than does the commonly used method of intramuscle injection of plasmid DNA.Author: Takeshi Suda, Dexi Liu
https://www.sciencedirect.com/science/article/pii/S0065266005540045
Hydrodynamic delivery has emerged as a near‐perfect method for intracellular DNA delivery in vivo. For gene delivery to parenchymal cells, only essential DNA sequences need to be injected via a selected blood vessel, eliminating safety concerns associated with current viral and synthetic vectors.
https://www.ncbi.nlm.nih.gov/pubmed/16724360
BACKGROUND: The hydrodynamic tail vein (HTV) injection of naked plasmid DNA is a simple yet effective in vivo gene delivery method into hepatocytes. It is increasingly being used as a research tool to elucidate mechanisms of gene expression and the role of genes and their cognate proteins in the pathogenesis of disease in animal models.Author: Magdolna G. Sebestyén, Vladimir G. Budker, Tatiana Budker, Vladimir M. Subbotin, Guofeng Zhang, Sean...
https://www.researchgate.net/publication/7063323_Mechanism_of_plasmid_delivery_by_hydrodynamic_tail_vein_injection_II_Morphological_studies
The efficient delivery of plasmid DNA (pDNA) to hepatocytes by a hydrodynamic tail vein (HTV) procedure has greatly popularized the use of naked nucleic acids.
https://www.researchgate.net/publication/10594392_Hydrodynamic_delivery_of_DNA
Hydrodynamic delivery is an efficient and inexpensive procedure to deliver a wide range of nucleic acids to hepatic tissues and other organs in vivo. The successful application of hydrodynamic delivery is dependent on the rapid injection of a large aqueous volume containing DNA, RNA or other molecules into the vasculature of the liver.
https://www.jove.com/video/51481/transient-expression-of-proteins-by-hydrodynamic-gene-delivery-in-mice
Hydrodynamic gene delivery allows the rapid analysis of gene expression in vivo. It requires the simple construction of the gene under study into a eukaryotic expression vector and the ability to perform the injection as outlined above.Author: Daniella Kovacsics, Jayne Raper
https://www.mirusbio.com/products/accessories/label-it-plasmid-delivery-controls
Trans IT®-QR Hydrodynamic Delivery Solution was used to deliver 25 µg of Label IT® Fluorescein Plasmid Control (green) to a mouse using hydrodynamic delivery via the tail vein. One hour post-injection the liver was harvested. Sections were fixed to stain the nuclei (blue) and the actin (red).
https://iacuc.ucsf.edu/sites/g/files/tkssra751/f/wysiwyg/HydrodynamicGeneDelivery_0.pdf
Hydrodynamic transfection is achieved by a large volume IV dose rapidly administered. The solution to be administered is prepared and kept at room temperature until injection -up to 10% of the animal’s body weight in volume can be administered per injection.
https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(18)30145-8
Sep 07, 2018 · Delivery of genes to mouse liver is routinely accomplished by tail-vein injections of viral vectors or naked plasmid DNA. While viral vectors are typically injected in a low-pressure and -volume fashion, uptake of naked plasmid DNA to hepatocytes is facilitated by high pressure and volumes, also known as hydrodynamic delivery.Author: Trine Dalsgaard, Claudia R. Cecchi, Anne Louise Askou, Rasmus O. Bak, Pernille O. Andersen, David Ho...
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