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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064722/
The mechanism of hydrodynamic delivery to liver hepatocytes has been well studied (9, 10, 21). Anatomically, a bolus injection of DNA solution into the tail vein induces immediate congestion in the heart, causing accumulation of the injected solution in the IVC and elevation of intravascular pressure in this venous section.Author: Barbara Bonamassa, Li Hai, Dexi Liu
https://www.sciencedirect.com/science/article/pii/S1525001616327198
The most successful application of the principles of hydrodynamic delivery is seen in gene delivery to hepatocytes in rodents. The standard procedure involves a tail vein injection in 5–7 seconds of physiological solution, equivalent to 8–10% of body weight. 2, 3 Mechanistically, a bolus tail vein injection of such a large volume of DNA solution that enters directly into the inferior vena ...Author: Takeshi Suda, Dexi Liu
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4096022/
The hydrodynamic tail vein injection is a technique that is used to deliver nucleic acids into live mice. Delivery through this method results in the in vivo transfection of foreign DNA primarily in the liver. Here, we describe the use of this technique ...Author: Mee J. Kim, Nadav Ahituv
https://www.jove.com/video/51481/transient-expression-of-proteins-by-hydrodynamic-gene-delivery-in-mice
Since its first description by Liu et al. and Zhang et al., hydrodynamic gene delivery (HGD) has become an invaluable tool for studying gene function in rodent model systems 1, 2.The technique involves the rapid injection (5-7 sec) of a large volume (8-12% body weight) of solution into the tail vein of mice to facilitate the uptake of plasmid DNA by the cells of target organs 1, 2.Author: Daniella Kovacsics, Jayne Raper
https://www.mirusbio.com/products/in-vivo/transit-ee-delivery-solution
TransIT®-QR or TransIT®-EE Delivery Solutions were used to deliver 10 µg of a luciferase expression vector to mice using the hydrodynamic delivery tail vein injection procedure. Twenty-four hours post-injection the livers were harvested and assayed for luciferase activity.
https://www.mirusbio.com/products/in-vivo/transit-qr-delivery-solution
TransIT®-QR Delivery Solution Effectively Delivers the Label IT® Cy®3 RNAi Delivery Control to Hepatocytes. TransIT®-QR Delivery Solution was used to deliver 25 µg of Label IT® Cy®3 RNAi Delivery Control (red) to a mouse using hydrodynamic delivery via the tail vein. Forty-five minutes post-injection the liver was harvested and sections were fixed then counterstained to stain the nuclei ...
https://www.cell.com/molecular-therapy-family/nucleic-acids/pdfExtended/S2162-2531(18)30145-8
gene delivery to combining these strategies and applied the hydrodynamic injection80% of hepatocytes after hydrodynamic vec-tor delivery. In summary, lentiviral vector transfer to mouse liver can be strongly augmented by hydrodynamic tail-vein in-jections, resulting in both reduced off-target delivery and transduction of the majority of ...
https://www.researchgate.net/publication/7664463_Hydrodynamic_Delivery
This is the first demonstration of somatic delivery and long-term expression of a genomic DNA transgene > 100 kb in vivo and shows that hydrodynamic tail vein injection can be used to deliver and ...
https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(18)30145-8
Sep 07, 2018 · Delivery of genes to mouse liver is routinely accomplished by tail-vein injections of viral vectors or naked plasmid DNA. While viral vectors are typically injected in a low-pressure and -volume fashion, uptake of naked plasmid DNA to hepatocytes is facilitated by high pressure and volumes, also known as hydrodynamic delivery. In this study, we compare the efficacy and specificity of delivery ...Author: Trine Dalsgaard, Claudia R. Cecchi, Anne Louise Askou, Rasmus O. Bak, Pernille O. Andersen, David Ho...
https://www.sciencedirect.com/science/article/pii/S0065266005540045
The principle of hydrodynamic delivery using large volume and rapid injection as the driving force for DNA delivery was established when Liu 1999, Zhang 1999 demonstrated highly efficient gene delivery in mice by a rapid injection via the tail vein of DNA solutions in volumes equivalent to 8–10% of the body weight. It was shown that, among ...
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