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https://www.nature.com/articles/nprot.2012.131
Nov 15, 2012 · Examples of anticipated results in which exosome-mediated siRNA delivery is evaluated by functional assays and imaging are also provided. The entire protocol takes ∼ 3 …Author: Samir El-Andaloussi, Samir El-Andaloussi, Yi Lee, Samira Lakhal-Littleton, Jinghuan Li, Yiqi Seow, C...
https://www.ncbi.nlm.nih.gov/pubmed/23154783
Exosome-mediated delivery of siRNA in vitro and in vivo. El-Andaloussi S(1), Lee Y, Lakhal-Littleton S, Li J, Seow Y, Gardiner C, Alvarez-Erviti L, Sargent IL, Wood MJ. Author information: (1)Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.Author: Samir El-Andaloussi, Samir El-Andaloussi, Yi Lee, Samira Lakhal-Littleton, Jinghuan Li, Yiqi Seow, C...
https://www.nature.com/articles/doi:10.1038/nprot.2012.131
Exosomes are promising tools for drug delivery across different biological barriers. Here we show how exosomes derived from cultured cells can be harnessed for delivery of siRNA in vitro and in vivo.Author: Samir El-Andaloussi, Samir El-Andaloussi, Yi Lee, Samira Lakhal-Littleton, Jinghuan Li, Yiqi Seow, C...
https://europepmc.org/article/MED/23154783
Nov 15, 2012 · Here we show how exosomes derived from cultured cells can be harnessed for delivery of siRNA in vitro and in vivo. This protocol first describes the generation of targeted exosomes through transfection of an expression vector, comprising an exosomal protein fused with a peptide ligand.Author: Samir El-Andaloussi, Samir El-Andaloussi, Yi Lee, Samira Lakhal-Littleton, Jinghuan Li, Yiqi Seow, C...
https://www.researchgate.net/publication/233423883_Exosome-mediated_delivery_of_siRNA_in_vitro_and_in_vivo
Request PDF Exosome-mediated delivery of siRNA in vitro and in vivo The use of small interfering RNAs (siRNAs) to induce gene silencing has opened a new avenue in drug discovery.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112038/
Aug 09, 2016 · The discovery that extracellular vesicles mediate the transport of functional small RNAs between cells has raised considerable interest for exosomes to serve as a novel delivery vehicle for therapeutic oligonucleotides. 36 Methods of loading oligonucleotides into exosomes have included electroporation 29,37 or overexpression of siRNA in exosome-producing cells. 38,39,40 Though …Author: Marie-Cécile Didiot, Lauren M Hall, Andrew H Coles, Reka A Haraszti, Bruno Mdc Godinho, Kathryn Chas...
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