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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC30628/
Mar 13, 2001 · Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O Iwan Walev , * Sebastian Chakrit Bhakdi , † Fred Hofmann , † Nabil Djonder , † Angela Valeva , * Klaus Aktories , † and Sucharit Bhakdi * ‡Author: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...
https://www.pnas.org/content/pnas/98/6/3185.full.pdf
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O Iwan Walev*, Sebastian Chakrit Bhakdi†, Fred Hofmann†, Nabil Djonder†, Angela Valeva*, Klaus Aktories†, and Sucharit Bhakdi*‡Author: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...
https://www.pnas.org/content/98/6/3185
Mar 13, 2001 · Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O Iwan Walev , Sebastian Chakrit Bhakdi , Fred Hofmann , Nabil Djonder , Angela Valeva , Klaus Aktories , and Sucharit BhakdiAuthor: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...
https://www.researchgate.net/publication/12084432_Delivery_of_proteins_into_living_cells_by_reversible_membrane_permeabilization_with_streptolysin-O
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O April 2001 Proceedings of the National Academy of Sciences 98(6):3185-90
https://ui.adsabs.harvard.edu/abs/2001PNAS...98.3185W/abstract
The presented method for introducing proteins into living cells should find multifaceted application in cell biology. The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174779
We have approached the development of a vector-/carrier-free method for intracellular delivery of a broad spectrum of cargos by combining exposure to a permeabilizing solution with modified strategies for both applying the permeabilizing solution to the cells and …Author: Shirley O'Dea, Valeria Annibaldi, Louise Gallagher, Joanne Mulholland, Emer L. Molloy, Conor J. Bree...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148600/
Dec 09, 2016 · Site-specific fluorescent labeling of proteins inside live mammalian cells has been achieved by employing Streptolysin O, a bacterial toxin which forms temporary pores in the membrane and allows delivery of virtually any fluorescent probes, ranging from labeled IgG’s to small ligands, with high efficiency (>85% of cells).Author: Kai Wen Teng, Yuji Ishitsuka, Pin Ren, Yeoan Youn, Xiang Deng, Pinghua Ge, Andrew S. Belmont, Paul R...
https://www.researchgate.net/publication/315876524_Vector-free_intracellular_delivery_by_reversible_permeabilization/fulltext/5a07ba444585157013a5d1b8/315876524_Vector-free_intracellular_delivery_by_reversible_permeabilization.pdf
Delivery of molecules into living cells is highly desirable for a wide range of both research and ... ing reversible cell membrane permeabilization. As others have done [11], we started with the ...
http://core.ac.uk/display/3162749
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O . By Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories and Sucharit Bhakdi. Cite . BibTex; Full citation ...
https://www.ahajournals.org/doi/10.1161/01.RES.77.2.220
In one experiment, three different concentrations of a fluorescein-labeled 11-mer (100 nmol/L, 1 μmol/L, and 10 μmol/L) were introduced into reversible permeabilization solutions 2 through 4 to determine whether the distribution of fluorescent ODNs was concentration dependent. This ODN was synthesized by Operon Technologies, Inc.
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