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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC30628/
Mar 13, 2001 · The presented method for introducing proteins into living cells should find multifaceted application in cell biology. Keywords: protein delivery, pore-forming toxin Methods for delivering proteins to the cytosol under retention of cell viability are scarce.Author: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...
https://www.pnas.org/content/98/6/3185
Mar 13, 2001 · Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O Iwan Walev , Sebastian Chakrit Bhakdi , Fred Hofmann , Nabil Djonder , Angela Valeva , Klaus Aktories , and Sucharit BhakdiAuthor: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...
https://www.pnas.org/content/pnas/98/6/3185.full.pdf
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O Iwan Walev*, Sebastian Chakrit Bhakdi†, Fred Hofmann†, Nabil Djonder†, Angela Valeva*, Klaus Aktories†, and Sucharit Bhakdi*‡Author: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...
https://www.researchgate.net/publication/12084432_Delivery_of_proteins_into_living_cells_by_reversible_membrane_permeabilization_with_streptolysin-O
The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol.
https://ui.adsabs.harvard.edu/abs/2001PNAS...98.3185W/abstract
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O - NASA/ADS The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148600/
Dec 09, 2016 · Site-specific fluorescent labeling of proteins inside live mammalian cells has been achieved by employing Streptolysin O, a bacterial toxin which forms temporary pores in the membrane and allows delivery of virtually any fluorescent probes, ranging from labeled IgG’s to small ligands, with high efficiency (>85% of cells).Author: Kai Wen Teng, Yuji Ishitsuka, Pin Ren, Yeoan Youn, Xiang Deng, Pinghua Ge, Andrew S. Belmont, Paul R...
https://www.nature.com/articles/nature19764
Oct 12, 2016 · Intracellular delivery to mammalian cells has become critical to both fundamental research applications and cell-based therapies.Author: Martin P. Stewart, Armon Sharei, Xiaoyun Ding, Gaurav Sahay, Robert Langer, Klavs F. Jensen
https://www.sciencedirect.com/science/article/pii/S0142961219304570
2. Strategies for cytosolic protein delivery. Generally, the strategies for cytosolic protein delivery include physical methods, membrane lytic or endosomolytic agents, cell penetrant peptides or analogues, and the use of nanoparticles, liposomes or polymers to facilitate protein transport across cell …Author: Jia Lv, Qianqian Fan, Hui Wang, Yiyun Cheng, Yiyun Cheng
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686919/
Jun 01, 2013 · The use of a number of small peptide tags has allowed for whole proteins to be drawn into cells without the addition of other agents to facilitate their import. TAT, antennepedia, and polyarginine are the best known sequences, and all three are cationic peptides that associate with the cell membrane prior to import.Author: Jeremy Ryan, Anthony Letai
https://advances.sciencemag.org/content/6/15/eaaz6980.full
3 days ago · As extracellular vesicles that play an active role in intercellular communication by transferring cellular materials to recipient cells, exosomes offer great potential as a natural therapeutic drug delivery vehicle. The inflammatory responses in various disease models can be attenuated through introduction of super-repressor IκB (srIκB), which is the dominant active form of IκBα and can ...
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