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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC30628/
Mar 13, 2001 · Reversible permeabilization of cells with SLO requires selection of the correct toxin dose. The permeabilization step is conducted in the absence of Ca 2+ , and resealing is induced by addition of Ca 2+ after 10–15 min. Resealing is temperature-independent and occurs effectively at 4°C.Author: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...
https://www.pnas.org/content/98/6/3185
Mar 13, 2001 · Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O Iwan Walev , Sebastian Chakrit Bhakdi , Fred Hofmann , Nabil Djonder , Angela Valeva , Klaus Aktories , and Sucharit BhakdiAuthor: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...
https://www.pnas.org/content/pnas/98/6/3185.full.pdf
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O Iwan Walev*, Sebastian Chakrit Bhakdi †, Fred Hofmann †, Nabil Djonder †, Angela Valeva*, Klaus Aktories †, and Sucharit Bhakdi* ‡Author: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...
https://ui.adsabs.harvard.edu/abs/2001PNAS...98.3185W/abstract
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O - NASA/ADS The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with …
https://www.researchgate.net/publication/12084432_Delivery_of_proteins_into_living_cells_by_reversible_membrane_permeabilization_with_streptolysin-O
The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol.
https://www.onlinelibrary.wiley.com/doi/full/10.1002/adhm.201701040
The human organism and its constituting cells rely on interplay between multiple proteins exerting specific functions. Progress in molecular biotechnologies has facilitated the production of recombinant proteins. When administrated to patients, recombinant proteins can …Author: Manuela Chiper, Karen Niederreither, Guy Zuber
https://www.sciencedirect.com/science/article/pii/S138917230570339X
Protein transduction, an efficient method for delivery of proteins into living cells, has many potential applications that range from basic study of protein function to development of novel therapeutics. Recent progress in protein transduction technology is promising for such purposes.Author: Junichiro Futami, Midori Kitazoe, Takashi Maeda, Emiko Nukui, Masakiyo Sakaguchi, Jun Kosaka, Masahi...
https://www.researchgate.net/publication/15530458_Delivery_of_proteins_into_cells_using_cationic_liposomes
We describe a procedure for delivery of purified proteins into a variety of tissue culture cells using a new polycationic lipid preparation, LipofectAMINE. Several different proteins, with diverse physical properties, can be delivered into cells by this method.
https://www.nature.com/articles/ncomms12277
Jul 22, 2016 · Purified EXPLORs can be used for delivery of the cargo proteins into target cells via membrane fusion or endocytosis processes.Author: Nambin Yim, Seung Wook Ryu, Kyungsun Choi, Kwang Ryeol Lee, Seunghee Lee, Hojun Choi, Jeongjin Kim, ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148600/
Dec 09, 2016 · Site-specific fluorescent labeling of proteins inside live mammalian cells has been achieved by employing Streptolysin O, a bacterial toxin which forms temporary pores in the membrane and allows delivery of virtually any fluorescent probes, ranging from labeled IgG’s to small ligands, with high efficiency (>85% of cells).Author: Kai Wen Teng, Yuji Ishitsuka, Pin Ren, Yeoan Youn, Xiang Deng, Pinghua Ge, Andrew S. Belmont, Paul R...
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