Delivery Of Proteins Into Living Cells

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Delivery of proteins into living cells by reversible ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC30628/
    Mar 13, 2001 · The presented method for introducing proteins into living cells should find multifaceted application in cell biology. Keywords: protein delivery, pore-forming toxin Methods for delivering proteins to the cytosol under retention of cell viability are scarce.Author: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...

Delivery of proteins into living cells by reversible ...

    https://www.pnas.org/content/98/6/3185
    Mar 13, 2001 · Inactivation of these G-proteins resulted in reduced stimulus-dependent granule secretion, whereas ADP-ribosylation of actin by the C. botulinum C2-toxin resulted in enhanced secretion in cells. The presented method for introducing proteins into living cells should find multifaceted application in cell biology.Author: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...

Delivery of proteins into living cells by reversible ...

    https://www.pnas.org/content/pnas/98/6/3185.full.pdf
    Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O Iwan Walev*, Sebastian Chakrit Bhakdi†, Fred Hofmann†, Nabil Djonder†, Angela Valeva*, Klaus Aktories†, and Sucharit Bhakdi*‡Author: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...

(PDF) Delivery of proteins into living cells by reversible ...

    https://www.researchgate.net/publication/12084432_Delivery_of_proteins_into_living_cells_by_reversible_membrane_permeabilization_with_streptolysin-O
    The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol.

Transduction Methods for Cytosolic Delivery of Proteins ...

    https://www.onlinelibrary.wiley.com/doi/full/10.1002/adhm.201701040
    To date, direct protein delivery is extremely tedious and inefficient in cultured cells, even more so in animal models of pathology. Novel techniques are changing this limitation, as recently developed in vitro methods can robustly convey large amount of proteins into cell cultures.Author: Manuela Chiper, Karen Niederreither, Guy Zuber

Intracellular delivery of proteins into mammalian living ...

    https://www.sciencedirect.com/science/article/pii/S138917230570339X
    For the third model protein, a 150-kDa immunoglobulin (IgG) was delivered into living cells by PEI-cationazation method . To demonstrate the functionality of intracellular protein delivery by PEI-cationization, we chose to examine visualization of antigen protein in living cell.Author: Junichiro Futami, Midori Kitazoe, Takashi Maeda, Emiko Nukui, Masakiyo Sakaguchi, Jun Kosaka, Masahi...

PEP and CADY-mediated delivery of fluorescent peptides and ...

    https://www.sciencedirect.com/science/article/pii/S0005273610000878
    PEP and CADY-mediated delivery of fluorescent peptides and proteins into living cells 1. Introduction: features and applications of cell-penetrating peptides. 2. Primary amphipathic PEP carriers: delivery of proteins, peptides and PNAs. 3. The secondary amphipathic carrier CADY: delivery of siRNA. ...Author: Laetitia Kurzawa, Morgan Pellerano, May C. Morris

Delivery of macromolecules into living cells: a method ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC51919/
    Jul 01, 1991 · Delivery of macromolecules into living cells: a method that exploits folate receptor endocytosis. C P Leamon and P S Low Department of Chemistry, Purdue …Author: Christopher P. Leamon, Philip Low

Delivery of antibody-captured proteins into living cells ...

    https://link.springer.com/article/10.1007/s10529-006-9076-9
    Jun 28, 2006 · Protein transduction domain (PTD)-mediated protein delivery into animal cells is a useful technique for regulating cellular functions. Proteins captured by antibodies were delivered into living cells using an antibody/PTD-fused protein A complex.Author: Masayasu Mie, Kazuto Mori, Hisakage Funabashi, Eiry Kobatake

BioPORTER® Protein Delivery Reagent amsbio

    http://www.amsbio.com/bioporter-protein-delivery-transfection-reagent.aspx
    Efficient delivery of proteins into living cells Efficient protein delivery into a wide variety of cell types; Efficient delivery of a variety of proteins, peptides, and other macromolecules; Fast and Easy Protocol; BioPORTER ® Protein Delivery Reagent is a unique lipid formulation that allows direct translocation of proteins into living cells.

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