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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924640/
Aug 20, 2010 · Over the past decade, a variety of reagents for the delivery of proteins into mammalian cells have been developed including lipid-linked compounds , nanoparticles , …Author: James J. Cronican, David B. Thompson, Kevin T. Beier, Brian R. McNaughton, Connie Cepko, David Ruchi...
https://www.cell.com/cell/fulltext/S0092-8674(15)00315-3
Apr 23, 2015 · Here, we describe a method for the efficient delivery of native proteins and other macromolecules, such as small RNAs, into primary cells. We discovered that a combination of small molecules drives the highly efficient intracellular delivery of native proteins, independent of any transduction peptide.Author: Diego S. D’Astolfo, Romina J. Pagliero, Anita Pras, Wouter R. Karthaus, Hans Clevers, Vikram Prasad,...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151968/
Oct 16, 2007 · In conclusion, we have presented a practical approach for protein delivery into live cells and we have demonstrated that the determination of optimal conditions is required for efficient delivery. Routinely, we recommend using 1–2 μg of R-PE and 2–4 μL of …Author: Claire O. Weill, Stéphanie Biri, Abdennaji Adib, Patrick Erbacher
https://www.nature.com/articles/nbt1201-1173
Dec 01, 2001 · A peptide carrier for the delivery of biologically active proteins into mammalian cells Skip to main content Thank you for visiting nature.com.Author: May C. Morris, Julien Depollier, Jean Mery, Frederic Heitz, Gilles Divita
https://www.researchgate.net/publication/15530458_Delivery_of_proteins_into_cells_using_cationic_liposomes
We describe a procedure for delivery of purified proteins into a variety of tissue culture cells using a new polycationic lipid preparation, LipofectAMINE.
https://www.pnas.org/content/98/6/3185
Mar 13, 2001 · Inactivation of these G-proteins resulted in reduced stimulus-dependent granule secretion, whereas ADP-ribosylation of actin by the C. botulinum C2-toxin resulted in enhanced secretion in cells. The presented method for introducing proteins into living cells should find multifaceted application in …Author: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...
https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/protein-delivery
An important feature of peptide-based protein delivery is that it allows for the relatively rapid introduction of proteins into cells. Using DNA-based transfection, a protein product is not usually observed until 8–24 h following transfection.
https://pubs.acs.org/doi/10.1021/jacs.8b10299
The efficient delivery of proteins into cells is needed to fully realize the potential of protein-based therapeutics. Current protein delivery strategies generally suffer from poor endosomal escape and low tolerance for serum. Here, the genetic fusion of a supercharged polypeptide, called SCP, to a protein provides a generic method for intracellular protein delivery. It allows efficient ...Author: Jun Yin, Qun Wang, Shan Hou, Lichen Bao, Wenbing Yao, Xiangdong Gao
https://pubs.acs.org/doi/abs/10.1021/cb1001153
An anionic human protein mediates cationic liposome delivery of genome editing proteins into mammalian cells. Nature Communications 2019, 10 (1) DOI: 10.1038/s41467-019-10828-3. Xiaofei Qin, Changmin Yu, Jing Wei, Lin Li, Chengwu Zhang, Qiong Wu, Jinhua Liu, Shao Q. Yao, Wei Huang.Author: James J. Cronican, David B. Thompson, Kevin T. Beier, Brian R. McNaughton, Connie Cepko, David Ruchi...
https://www.jove.com/video/53175/delivery-proteins-peptides-or-cell-impermeable-small-molecules-into
The delivery of proteins, peptides or cell-impermeable small molecules into live cells is often desirable in many biological or biotechnological applications (cellular imaging, functional assays, cellular reprogramming, etc.) 1-4.Author: Kristina Najjar, Alfredo Erazo-Oliveras, Jean-Philippe Pellois
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