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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC30628/
Mar 13, 2001 · The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10 5 –10 6 molecules were estimated to be entrapped per cell.Author: Iwan Walev, Sebastian Chakrit Bhakdi, Fred Hofmann, Nabil Djonder, Angela Valeva, Klaus Aktories, Su...
https://www.cell.com/cell/fulltext/S0092-8674(15)00315-3
Apr 23, 2015 · Here, we describe a method for the efficient delivery of native proteins and other macromolecules, such as small RNAs, into primary cells. We discovered that a combination of small molecules drives the highly efficient intracellular delivery of native proteins, independent of any transduction peptide.Author: Diego S. D’Astolfo, Romina J. Pagliero, Anita Pras, Wouter R. Karthaus, Hans Clevers, Vikram Prasad,...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900815/
Feb 19, 2016 · The delivery of proteins and other macromolecules to an intracellular site is made difficult by cellular membranes. 1 Extensive efforts have led to the development of effective delivery systems that invoke cell-penetrating peptides, 2–5 antibodies, 6 ligands for natural receptors, 7 dendrimers, 8 functionalized polymers, 9,10 liposomes, 11 or nanoparticles. 12,13 Extant strategies …Author: Kristen A. Andersen, Thomas P. Smith, Jo E. Lomax, Ronald T. Raines
https://www.portlandpress.com/biochemsoctrans/article/doi/10.1042/BST20190039/222602/Methods-for-protein-delivery-into-cells-from
The manipulation of cultured mammalian cells by the delivery of exogenous macromolecules is one of the cornerstones of experimental cell biology. Although the transfection of cells with DNA expressions constructs that encode proteins is routine and simple to perform, the direct delivery of proteins into cells has many advantages. For example, proteins can be chemically modified, assembled into ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924640/
Aug 20, 2010 · Over the past decade, a variety of reagents for the delivery of proteins into mammalian cells have been developed including lipid-linked compounds , nanoparticles , and fusions to receptor ligands …Author: James J. Cronican, David B. Thompson, Kevin T. Beier, Brian R. McNaughton, Connie Cepko, David Ruchi...
https://www.nature.com/articles/nbt1201-1173
Dec 01, 2001 · A series of small protein domains, termed protein transduction domains (PTDs), have been shown to cross biological membranes efficiently and …Author: May C. Morris, Julien Depollier, Jean Mery, Frederic Heitz, Gilles Divita
https://onlinelibrary.wiley.com/doi/full/10.1002/anie.201711651
Intracellular delivery of therapeutic proteins is highly challenging and in most cases requires chemical or genetic modifications. Herein, two complementary approaches for endocytosis‐independent delivery of proteins to live mammalian cells are reported.Author: Linghui Qian, Jiaqi Fu, Peiyan Yuan, Shubo Du, Wei Huang, Lin Li, Shao Q. Yao
https://pubs.acs.org/doi/10.1021/jacs.8b10947
Imaging studies demonstrate that the polymer–protein conjugates are successfully delivered into the cytosol of various cancer cell lines, likely through a membrane fusion mechanism. When conjugated to the delivery polymers, the activity of modified RNase A was retained and notably promoted cytotoxicity in cancer cells upon delivery to the cytosol.Author: Rapeepat Sangsuwan, Phum Tachachartvanich, Matthew B. Francis
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151968/
Oct 16, 2007 · Protein delivery procedure. Cells were plated in order to reach approximately 70–80% confluency the day of protein delivery experiment. For one well of a 24-well plate, 0.5–8 μg of purified protein was diluted in 100 μL of Hepes buffer (20 mM, pH 7.4) in a 1.5 mL microcentrifuge tube, under sterile conditions.Author: Claire O. Weill, Stéphanie Biri, Abdennaji Adib, Patrick Erbacher
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801296/
Mar 15, 2016 · The delivery of these proteins into HeLa-DsRed cells was evaluated by treating cells with 25 nM lipid/protein nanoparticles for 6 h to quantify the cellular uptake of protein or 24 h to analyze the DsRed protein expression profile.Author: Ming Wang, John Zuris, Fantao Meng, Holly Anne Rees, Shuo Sun, Pu Deng, Yong Han, Xue Gao, Dimitra P...
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