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https://www.cell.com/trends/biotechnology/fulltext/S0167-7799(01)01911-4
Apr 01, 2002 · The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. Recently, recombinant baculovirus vectors engineered to contain mammalian cell-active promoter elements, have been used successfully for transient and stable gene delivery in a broad spectrum of primary and established mammalian cells.Author: Thomas A. Kost, J.Patrick Condreay
https://www.cell.com/molecular-therapy-family/molecular-therapy/pdf/S1525-0016(16)32133-5.pdf
Efficient Gene Delivery and Selective Transduction of Glial Cells in the Mammalian Brain by AAV Serotypes Isolated From Nonhuman Primates Patricia A Lawlor1, Ross J Bland2, Alexandre Mouravlev 1, Deborah Young1,3 and Matthew J During
https://www.ncbi.nlm.nih.gov/pubmed/9829538
Nov 01, 1998 · In an effort to develop novel methods for ligand discovery via phage gene delivery, we conferred mammalian cell tropism to filamentous bacteriophages by attaching basic fibroblast growth factor (FGF2), transferrin, or epidermal growth factor (EGF) to their coat proteins and measuring CMV promoter-driven reporter gene expression in target cells.Author: David Larocca, Alison Witte, Wendy Johnson, Glenn F. Pierce, Andrew Baird
https://www.sciencedirect.com/science/article/pii/S0147619X18300301
However, baculovirus can also be harnessed for efficient gene delivery to mammalian cells if it is equipped with mammalian promoters. This technology is known as BacMam and has been used for gene delivery to immortalized cell lines, stem cells, and primary cells, as well as for gene delivery in animals.Author: Maysam Mansouri, Maysam Mansouri, Philipp Berger
http://cshprotocols.cshlp.org/content/2019/11/pdb.top095406.full
whether gene expression or protein production is the desired end result. the cell type to be used (adherent vs. suspension, culture-adapted vs. primary cells) the ability of the cell line to survive the stress of transfection. the type of assay to be used for screening. the culture conditionsAuthor: Priti Kumar, Arvindhan Nagarajan, Pradeep D. Uchil
https://www.thermofisher.com/kr/en/home/life-science/protein-biology/protein-expression/mammalian-protein-expression/viral-delivery-mammalian-expression.html
Whether you’re using a hard-to-transfect mammalian cell line, an animal model, or simply want efficient gene delivery, our ViraPower™ product portfolio is the ideal choice for …
https://link.springer.com/protocol/10.1385/1-59259-649-5:167
Barber (1, 2) first described the technique that forms the basis for today’s microinjection applications. Described here is a simple method for microinjecting individual adherent cells in culture. This application may be used for microinjecting the myriad mammalian cell types that may be encountered in this field.Author: Robert King
https://www.amazon.com/Gene-Delivery-Mammalian-Cells-Techniques/dp/1617373532
Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.Price: $11
https://www.nature.com/articles/s41467-020-15061-x
Mar 10, 2020 · Cell-killing gene circuits could be used for cancer gene therapies or cell therapies 20,21. However, sophisticated apoptosis regulation has remained a challenge due to leakiness of the protein ...Author: Hideyuki Nakanishi, Hideyuki Nakanishi, Hirohide Saito
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-expression-handbook/pex-handbook-mammalian-cell-based-protein-expression.html
You can introduce your gene of interest into mammalian cells by transfecting the plasmid DNA using chemical reagents, by electroporation, or by viral transduction using recombinant viruses. The choice of delivery method depends upon the mammalian cell type that is …
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