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https://www.nature.com/articles/nchem.2779
May 22, 2017 · One of the major obstacles in intracellular targeting using antibodies is their limited release from endosomes into the cytosol. Here we report …Author: Misao Akishiba, Toshihide Takeuchi, Yoshimasa Kawaguchi, Kentarou Sakamoto, Hao-Hsin Yu, Ikuhiko Nak...
https://www.nature.com/articles/s41598-019-55091-0
Dec 10, 2019 · Intracellular delivery of therapeutic antibodies into specific cells using antibody-peptide fusions Skip to main content Thank you for visiting nature.com.Author: Julie Gaston, Nicolas Maestrali, Guilhem Lalle, Marie Gagnaire, Alessandro Masiero, Bruno Dumas, Tar...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171028/
Jul 01, 2014 · In order to assess whether scFv-Fc fusions are also stable and functional in the reducing environment of the cytosol, a series of dilutions of the anti-Myosin (SF9) scFv-Fc antibody were electroporated into living HeLa cells. Myosin binding was analyzed later using a secondary antibody …Author: Andrea Lj Marschall, Congcong Zhang, André Frenzel, Thomas Schirrmann, Michael Hust, Franck Perez, S...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207587/
Oct 24, 2018 · All these physical delivery methods offer the advantage of direct antibody delivery inside the cytosol with the ability to target other compartments (e.g., the nucleus) upon the attachment of the specific localization signal, as shown for the nuclear delivery of anti-PCNA antibody conjugates with NLS delivered into the cells via electroporation ...Author: Tatiana A. Slastnikova, A. V. Ulasov, A. A. Rosenkranz, A. A. Rosenkranz, A. S. Sobolev, A. S. Sobol...
https://www.sciencedirect.com/science/article/pii/S232905012030019X
Rapid Delivery of Nanobodies/V H Hs into Living Cells via Expressing In Vitro-Transcribed mRNA. ... we presented a novel approach to rapidly deliver the nanobody/variable domain of heavy chain of heavy-chain antibody (V H H) into living cells via introducing its …Author: Xuechen Zhou, Rui Hao, Chen Chen, Zhipeng Su, Linhong Zhao, Zhuojuan Luo, Wei Xie
https://phys.org/news/2019-11-easier-antibodies-cells.html
Nov 05, 2019 · There, they will have to develop new delivery vehicles that are capable of getting the antibodies to their target cells in the first place, but they will also have an opportunity to tackle "the ...
https://www.antibodysociety.org/overcoming-antibody-delivery-challenges/
Sep 07, 2017 · The final three talks will all approach the formidable problem of protein delivery, across the plasma membrane into the cytosol of cells. Wouter Verdurmen (Radboud University Medical Center, Nijmegen, The Netherlands) and collaborators developed a biotin ligase assay to quantify the relative efficiencies of various transport systems. This assay ...
https://www.nih.gov/news-events/news-releases/drug-delivery-technology-leads-sustained-hiv-antibody-production-nih-study
Mar 09, 2020 · This drug-delivery technology uses a harmless virus to deliver an antibody gene into human cells, enabling the body to generate the antibody over an extended time. With further development, such a strategy could be applied to prevent and treat a wide variety of infectious diseases, according to the study investigators.
https://pubs.acs.org/doi/pdf/10.1021/acsabm.9b00876
Protein and Antibody Delivery into Difficult-to-Transfect Cells by Polymeric Peptide Mimics Coralie M. Backlund,†,⊥ Christopher R. Hango,†,⊥ Lisa M. Minter,‡,§ and Gregory N. Tew*,†,‡,§ †Department of Polymer Science & Engineering, University of Massachusetts, Amherst, Massachusetts 01003, United States ‡Molecular and Cellular Biology Program, University of Massachusetts ...Author: Coralie M. Backlund, Christopher R. Hango, Lisa M. Minter, Gregory N. Tew
https://pubs.acs.org/doi/10.1021/acsabm.9b00876
Cell-penetrating peptides (CPPs) have the ability to efficiently internalize into cells and thus have been used as a vector for the intracellular delivery of various bioactive mols. The introduction of a hydrophobic core to CPPs may increase their interaction with membranes and facilitate their translocation into cells.Author: Coralie M. Backlund, Christopher R. Hango, Lisa M. Minter, Gregory N. Tew
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