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https://www.alliedacademies.org/articles/rethinking-delivery-viral-vectors-and-rna-interference-7646.html
These shRNAs are processed by the cellular machinery into siRNAs, which then knockdown the target gene. This strategy not only allows prolonged silencing, as the shRNAs are continuously produced, it also opens up the possibility of using viral vectors for the delivery of double-stranded RNA molecules.Author: Jens Kurreck
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Viral and nonviral delivery systems for gene delivery. ... There are different viral and nonviral vectors for gene delivery, but all gene therapy applications depend on the fact that the genetic material needs to be delivered across the cell membrane and ultimately to the cell nucleus. ... siRNA, dsRNA, shRNA, mRNA, ODN,…), nonviral ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2692493/
Jun 01, 2009 · Non-viral vectors for siRNA delivery. Of primary consideration in deciding on a drug delivery system for siRNA is whether the intended disease target lends itself to systemic or local administration. In the case of delivery of DNA encoding for the short hairpin RNA (shRNA) by non-viral delivery systems, nuclear translocation of the DNA is often ...Author: Kun Gao, Leaf Huang
https://www.thermofisher.com/us/en/home/life-science/rnai/delivering-rnai-to-cells-transfection-and-viral-delivery.html.html
For cell types not amenable to lipid-mediated transfection, viral vectors are often employed. Adenoviral vectors work well for transient delivery in many cell types; however, for some difficult cell lines, such as non-dividing cells and for stable RNAi expression, lentiviral vectors are the best delivery method.
https://www.nature.com/articles/nrg3763
Jul 15, 2014 · Synthetic delivery vectors have the potential to address many of the limitations of viral vectors, particularly with respect to safety. For systemic delivery of DNA, both lipid-based vectors and ...Author: Hao Yin, Rosemary L. Kanasty, Ahmed A. Eltoukhy, Arturo J. Vegas, Arturo J. Vegas, J. Robert Dorkin,...
http://altogenlabs.com/rna-interference-rnai-services/in-vivo-sirna-delivery-and-tissue-targeting/
Two basic methods are adapted for RNAi use in vivo: administration of siRNA or miRNA and administration of plasmid DNA and viral vectors, which in vivo express a short hairpin RNA (shRNA) that is subsequently processed into active siRNA. In general, viral-based shRNA vectors provide the highest delivery efficiency in vivo, but are time ...
https://www.sciencedirect.com/science/article/pii/S1742706117303641
Highly antibacterial polypeptide-based amphiphilic copolymers as multifunctional non-viral vectors for enhanced intracellular siRNA delivery and anti-infection. ... has been widely used as the hydrophilic segments in siRNA delivery vectors. PEG could enhance NP stability in physiological environment and protect siRNA from nuclease ...Author: Li Zhou, Yuewei Xi, Meng Yu, Min Wang, Yi Guo, Peng Li, Peter X. Ma, Bo Lei
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