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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949849/
Sep 21, 2010 · Viral delivery of shRNA expression cassettes. Combining RNAi with viral gene therapy vectors is a powerful approach in certain scenarios where spatiotemporal control over gene silencing is highly critical, and/or where persistent suppression of a target is mandatory for success.Author: Katja Sliva, Barbara S Schnierle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559924/
Jun 25, 2006 · To overcome these barriers several strategies have been developed, such as chemical modifications of siRNA, viral nucleic acid delivery systems, and nonviral nucleic acid delivery systems. Here, we discuss progress that has been made to improve targeted delivery of …Author: Sabrina Oliveira, Gert Storm, Raymond M. Schiffelers
https://www.alliedacademies.org/articles/rethinking-delivery-viral-vectors-and-rna-interference-7646.html
Viral vectors are a promising alternative to deliver short hairpin RNA (shRNA) expression cassettes. Many corporate researchers are reluctant to consider viral vectors as a means of delivery due to the poor reputation of gene therapy, as a consequence of adverse events that occurred in early studies.Author: Jens Kurreck
https://www.researchgate.net/publication/46392655_Selective_gene_silencing_by_viral_delivery_of_short_hairpin_RNA
Selective gene silencing by viral delivery of short hairpin RNA Article · Literature Review (PDF Available) in Virology Journal 7(1):248 · September 2010 with 81 Reads How we measure 'reads'
https://www.sciencedirect.com/science/article/pii/S0169409X09000969
Non-viral polymeric delivery systems, in particular those with biodegradable components, have much better safety profiles than their viral counterparts though their transfection efficiency is generally lower , , . Non-viral vehicles for delivery of siRNA and shRNA are typically cationic preparations.Author: Donald D. Rao, John S. Vorhies, Neil Senzer, John Nemunaitis
https://www.intechopen.com/books/rna-interference/non-viral-sirna-and-shrna-delivery-systems-in-cancer-therapy
Therefore, the development of siRNA-based viral and non-viral delivery systems are required to have an enhanced efficacy, improved stability, and minimized non-specific gene silencing such as off-target effects and immune responses. This chapter focuses on recent improvements in the non-viral siRNA delivery systems in cancer therapy.Author: Emine Şalva, Ceyda Ekentok, Suna Özbaş Turan, Jülide Akbuğa
https://www.thermofisher.com/us/en/home/life-science/rnai/virus-based-rnai-analysis/lentiviral-shrna-and-mirna-analysis.html
13 rows · Viral delivery can be the best option in virtually any mammalian cell type, including hard-to …
https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-7-248
Sep 21, 2010 · Viruses and the vectors derived from them carry out precisely this task and have become a major delivery system for shRNA. Here, we summarize and compare different currently used viral delivery systems, give examples of in vivo applications, and indicate trends for new developments, such as replicating viruses for shRNA delivery to cancer cells.Author: Katja Sliva, Barbara S Schnierle
https://www.thermofisher.com/us/en/home/life-science/rnai/delivering-rnai-to-cells-transfection-and-viral-delivery.html.html
Viral delivery can also be used to create stable cell lines with inducible RNAi expression or to express RNAi sequences with tissue-specific promoters. Importance of minimizing transfection-mediated cytotoxicity. The delivery of RNAi reagents, or the delivery method itself, can give rise to cytotoxicity in gene silencing experiments.
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