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https://www.alliedacademies.org/articles/rethinking-delivery-viral-vectors-and-rna-interference-7646.html
Viral vectors are a promising alternative to deliver short hairpin RNA (shRNA) expression cassettes. Many corporate researchers are reluctant to consider viral vectors as a means of delivery due to the poor reputation of gene therapy, as a consequence of adverse events that occurred in early studies.Author: Jens Kurreck
https://www.thermofisher.com/us/en/home/life-science/rnai/delivering-rnai-to-cells-transfection-and-viral-delivery.html
Delivering RNAi to Cells - Transfection and Viral Delivery. The two common approaches for RNAi delivery are lipid-mediated transfection and viral-mediated transduction. Determining which one of these approaches to use depends on the cell type being studied and whether transient or stable knockdown is desired (Tables 1 & 2).
https://www.thermofisher.com/us/en/home/life-science/rnai/virus-based-rnai-analysis/lentiviral-shrna-and-mirna-analysis.html
13 rows · Viral delivery of RNAi vectors is a powerful alternative to transfection for these cell types as …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949849/
Sep 21, 2010 · Viral delivery of shRNA expression cassettes. Combining RNAi with viral gene therapy vectors is a powerful approach in certain scenarios where spatiotemporal control over gene silencing is highly critical, and/or where persistent suppression of a target is mandatory for success.Author: Katja Sliva, Barbara S Schnierle
https://genomemedicine.biomedcentral.com/articles/10.1186/s13073-017-0450-0
Jun 27, 2017 · Broadly speaking, RNA delivery can be mediated by viral and non-viral vectors. For viral RNA delivery, there has been a great deal of interest in engineering adeno-associated viruses to carry nucleic acid cargo —however, this section will focus mainly on the development of non-viral materials (Table 1). Of the non-viral RNA delivery vehicles, nanoparticles are perhaps the most …Author: James C. Kaczmarek, Piotr S. Kowalski, Daniel G. Anderson
https://www.pnas.org/content/109/36/14604
Sep 04, 2012 · There has been extensive work on viral delivery of self-amplifying RNA using viral replicon particles (VRPs) (4, 11, 21 ⇓ ⇓ – 24). VRPs are potent vaccines in mice (10, 11), nonhuman primates (11, 22), and humans (25). These single-cycle alphavirus vectors were used as the viral delivery benchmark in our studies.Author: Andrew J. Geall, Ayush Verma, Gillis R. Otten, Christine A. Shaw, Armin Hekele, Kaustuv Banerjee, Ye...
https://www.sciencedirect.com/science/article/pii/S2214574518301482
Cross-kingdom RNAi: delivery by microbes Bacteria. Trigger RNA has been expressed in recombinant Escherichia coli for many years, and is often subsequently fed to target insects to elicit RNAi; however, in most insects E. coli can also stimulate an immune response.Author: Miranda Ma Whitten
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2977003/
Jun 11, 2010 · Due to the similar physicochemical properties between DNA and siRNA, carriers developed for DNA have also been applied to siRNA. These carriers can be broadly divided into two categories, i.e., viral and non-viral. Readers are referred to a recent review on viral delivery systems for RNAi . In part due to the potential toxicities associated with viral vectors, non-viral vectors have …Author: Jie Wang, Ze Lu, M. Guillaume Wientjes, Jessie L.-S. Au
https://www.sciencedirect.com/science/article/pii/S0168365915302832
A chemically diverse suite of delivery materials with origins as materials for cellular transfection of DNA and small interfering RNAs (siRNAs) has recently been reported to have promise as non-viral delivery agents for mRNA.Author: Kevin J. Kauffman, Matthew J. Webber, Matthew J. Webber, Daniel G. Anderson
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