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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2692493/
Jun 01, 2009 · Of primary consideration in deciding on a drug delivery system for siRNA is whether the intended disease target lends itself to systemic or local administration. In the case of delivery of DNA encoding for the short hairpin RNA (shRNA) by non-viral delivery systems, nuclear translocation of the DNA is often inadequate.Author: Kun Gao, Leaf Huang
https://www.thermofisher.com/us/en/home/life-science/rnai/delivering-rnai-to-cells-transfection-and-viral-delivery.html.html
A powerful alternative to cationic lipid-mediated transfection is viral delivery of vectors expressing RNAi sequences. This option is best for delivery to hard-to-transfect, primary, and nondividing cells.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559924/
Jun 25, 2006 · Delivery of chemically synthesized siRNA In this approach, reconstituted viral envelopes derived from influenza virus are used to encapsulate and deliver siRNAs. The reconstituted membrane vesicles contain the influenza virus spike protein hemagglutinin and additionally added cationic lipids.Author: Sabrina Oliveira, Gert Storm, Raymond M. Schiffelers
https://www.alliedacademies.org/articles/rethinking-delivery-viral-vectors-and-rna-interference-7646.html
In 2002, several groups independently developed systems to express short double-stranded RNA molecules intracellular instead of transfecting chemically synthesized siRNAs (Shi, 2003). These shRNAs are processed by the cellular machinery into siRNAs, which then knockdown the target gene. This strategy not only allows prolonged silencing, as the shRNAs are continuously produced, it also opens up the possibility of using viral vectors for the delivery …Author: Jens Kurreck
https://www.intechopen.com/books/rna-interference/non-viral-sirna-and-shrna-delivery-systems-in-cancer-therapy
Non-viral delivery systems have great potential for safe and effective delivery of siRNA therapeutics to tumor cells. Nanocarriers such as nanoplexes, lipoplexes, nanoparticles, and liposomes have been commonly used for siRNA delivery. This chapter highlights the importance of non-viral delivery systems in vitro and in vivo cancer therapy.Author: Emine Şalva, Ceyda Ekentok, Suna Özbaş Turan, Jülide Akbuğa
http://altogenlabs.com/rna-interference-rnai-services/in-vivo-sirna-delivery-and-tissue-targeting/
In Vivo siRNA Delivery and Tissue Targeting RNAi-induced gene silencing is used to study gene function in cultured cells ( in vitro ). Most often, these effects are induced by the introduction of small-interfering RNA (siRNA) or microRNA (miRNA).
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