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https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.200604295
Special delivery: Functionalized single‐walled nanotubes (SWNT) can be used as molecular transporters to shuttle short interfering RNA (siRNA) into human T cells and primary cells and silence the expression of HIV‐specific cell‐surface receptors and coreceptors (see picture; scale bars 40 μm). This silencing effect, known to block HIV viral entry and reduce infection, is superior to that observed with …Author: Zhuang Liu, Mark Winters, Mark Holodniy, Hongjie Dai
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2943428/
Aug 22, 2008 · In a therapeutic setting, delivery to naïve/resting T cells will be important to ensure that siRNA is present in cells at the time of activation when they become most vulnerable to infection. This is also important for controlling viral resurgence in latently infected memory T cells.Author: Priti Kumar, Hong Seok Ban, Sang Soo Kim, Haoquan Wu, Todd Pearson, Dale L. Greiner, Amale Laouar, J...
https://www.sciencedirect.com/science/article/pii/S0092867408008210
Aug 22, 2008 · In a therapeutic setting, delivery to naive and/or resting T cells will be important to ensure that siRNA is present in cells at the time of activation when they become most vulnerable to infection. This is also important for controlling viral resurgence in latently infected memory T cells.Author: Priti Kumar, Hong Seok Ban, Sang Soo Kim, Haoquan Wu, Todd Pearson, Dale L. Greiner, Amale Laouar, J...
https://www.sigmaaldrich.com/technical-documents/articles/biology/sirna-difficult-cells.html
The siRNA is a 19- to 25-bp dsRNA that can activate the RISC enzyme. The crucial factor in using siRNA is the successful delivery of the siRNA oligonucleotides to cultured cells. The siRNAs are often delivered into cells using transfection reagents; however, efficient siRNA delivery is difficult in some cell …
https://onlinelibrary.wiley.com/doi/full/10.1002/wnan.1634
Polyplexes made from siRNA complexed with Tf‐PEI and Mel‐PEI are shown to be specifically taken up by ATCs facilitating endosomal release necessary for efficient gene silencing. By spray drying, dry ...
https://www.nature.com/articles/nbt.1564
Sep 13, 2009 · In vivo delivery of siRNA to immune cells by conjugation to a TLR9 agonist enhances antitumor immune responses Abstract. Efficient delivery of small interfering... Discussion. We have developed a strategy that combines targeted siRNA delivery and activation... Methods. Murine B16 melanoma, CT26 ...Author: Marcin Kortylewski, Piotr Swiderski, Andreas Herrmann, Lin Wang, Claudia Kowolik, Maciej Kujawski, H...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2694965/
PTD-DRBD siRNA delivery into T cells and HUVECs Primary human umbilical vein endothelial cells (HUVEC) are an important cell type for large scale RNAi screen; however, lipofection delivery of HUVECs results in both poor siRNA delivery and cytotoxicity. We targeted endogenous GAPDH mRNA by PTD-DRBD mediated RNAi.Author: Akiko Eguchi, Akiko Eguchi, Akiko Eguchi, Bryan R Meade, Bryan R Meade, Yung-Chi Chang, Craig T Fred...
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