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https://www.biorxiv.org/content/10.1101/564427v1
Mar 01, 2019 · Post-transcriptional gene silencing (PTGS) is a powerful tool to understand and control plant metabolic pathways, which is central to plant biotechnology. PTGS is commonly accomplished through delivery of small interfering RNA (siRNA) into cells. While siRNA delivery has been optimized for mammalian systems,...Author: Gozde Demirer, Huan Zhang, Natalie Goh, Roger Chang, Markita Landry
https://papers.ssrn.com/sol3/papers.cfm?abstract_id=3352632
Apr 07, 2019 · PTGS is accomplished through delivery of small interfering RNA (siRNA) into cells, which has been optimized for mammalian systems but remains a significant challenge for plants due to the plant cell wall. Standard plant siRNA delivery methods (Agrobacterium and viruses) involve coding siRNA into DNA vectors, and are only tractable for certain plant species.Author: Gozde Demirer, Huan Zhang, Natalie Goh, Roger Chang, Markita Landry
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542916/
Mar 30, 2017 · The siRNA delivery systems are categorized as non-viral and viral delivery systems. The non-viral delivery system includes polymers; Lipids; peptides etc. are the widely studied delivery systems for siRNA. Effective pharmacological use of siRNA requires ‘carriers’ that can deliver the siRNA to its intended site of action.Author: Hassan Dana, Ghanbar Mahmoodi Chalbatani, Habibollah Mahmoodzadeh, Rezvan Karimloo, Omid Rezaiean, A...
https://www.biorxiv.org/content/10.1101/722595v1
Utilizing a class of very small nanoparticles called carbon dots, a method of delivering siRNA into the model plant Nicotiana benthamiana and tomato is described. Low-pressure spray application of these formulations with a spreading surfactant resulted in strong silencing of GFP transgenes in both species.Author: Steven H Schwartz, Bill Hendrix, Paul Hoffer, Richard A Sanders, Wei Zheng
https://www.pnas.org/content/116/15/7543
Apr 09, 2019 · Plant bioengineering will be necessary to sustain plant biology and agriculture, where the delivery of biomolecules such as DNA, RNA, or proteins to plant cells is at the crux of plant biotechnology. Here, we show that DNA nanostructures can internalize into plant cells and deliver siRNA to mature plant tissues without external aid.Author: Huan Zhang, Gozde S. Demirer, Honglu Zhang, Tianzheng Ye, Natalie S. Goh, Abhishek J. Aditham, Franc...
https://www.slideshare.net/IshaqueAbdulla/si-rna
Nov 19, 2013 · These delivery me-thods are being used to facilitate therapeutic applications employing siRNA. siRNA can be applied to the treatment of viral and genetic diseases and cancer. 15. • RNA interference is a vital part of the immune response to viruses and other foreign genetic material, especially in plants where it may also prevent the self-propagation of transposons.
https://www.nature.com/articles/nplants2016207
Jan 09, 2017 · Topical application of pathogen-specific double-stranded RNA (dsRNA) for virus resistance in plants represents an attractive alternative to …Author: Neena Mitter, Elizabeth A. Worrall, Karl E. Robinson, Peng Li, Ritesh G. Jain, Christelle Taochy, St...
https://www.nature.com/articles/s41573-019-0017-4
Mar 07, 2019 · Castleberry, S. A. et al. Nanolayered siRNA delivery platforms for local silencing of CTGF reduce cutaneous scar contraction in third-degree burns. …Author: Ryan L. Setten, Ryan L. Setten, John J. Rossi, John J. Rossi, Si-ping Han, Si-ping Han
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2427156/
Therefore the purpose of our current study was to compare and contrast siRNA uptake in vivo utilizing one of four delivery methods: 1) hydrodynamic IV tail vein injection, 2) standard IV tail vein injection, 3) intraperitoneal (IP) injection and 4) rectal administration.Author: Shawn D. Larson, Lindsey N. Jackson, L. Andy Chen, Piotr G. Rychahou, B. Mark Evers
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