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https://www.nature.com/articles/nplants2016207
Jan 09, 2017 · Topical application of pathogen-specific double-stranded RNA (dsRNA) for virus resistance in plants represents an attractive alternative to …Author: Neena Mitter, Elizabeth A. Worrall, Karl E. Robinson, Peng Li, Ritesh G. Jain, Christelle Taochy, St...
https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.12662
Sep 16, 2014 · Systemic delivery of siRNA in pumpkin by a plant PHLOEM SMALL RNA‐BINDING PROTEIN 1–ribonucleoprotein complex. Byung‐Kook Ham. Department of Plant Biology, College of Biological Sciences, University of California, Davis, CA, 95616 USA. Search for more papers by …
http://landrylab.com/wp-content/uploads/2019/03/564427.full_.pdf
for plants due to the plant cell wall. Standard plant siRNA delivery methods (Agrobacterium and viruses) involve coding siRNA into DNA vectors, and are only tractable for certain plant species. Herein, we develop a nanotube-based platform for direct delivery of siRNA, and show high silencing efficiency in intact plant cells. We
https://onlinelibrary.wiley.com/doi/full/10.1002/jgm.3097
Targeted delivery has its advantages of keeping effective on a low dosage level and reducing the side effects with respect to unintended tissues. 22 GalNAc‐siRNA conjugate (Figure 2C) is a representative targeting delivery strategy as a result of its outstanding siRNA transporting ability and …
https://www.biorxiv.org/content/10.1101/722595v1
The Initiation of RNA interference (RNAi) by topically applied double stranded RNA (dsRNA) has potential applications for plant functional genomics, crop improvement and crop protection. The primary obstacle for the development of this technology is efficient delivery of RNAi effectors. The plant cell wall is a particularly challenging barrier to the delivery of macromolecules. Many of the ...
https://www.researchgate.net/publication/332283350_Nanotubes_Effectively_Deliver_siRNA_to_Intact_Plant_Cells_and_Protect_siRNA_Against_Nuclease_Degradation
delivery is the preferred method to deliver siRNA into int act plant cells. Viral vectors present the advantage of directly and strongly expressing the siRNA without relying on
https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-10-291
Dec 30, 2010 · Recently, siRNA delivery via viral vectors (Potato virus X, Barley stripe mosaic virus) to intact N. benthamiana and barley plants has provided valuable evidence that RNAi technology can be employed to knockdown CesA-1, CesA-2, and CesA-6 gene expression [25, 44]. By silencing individual members of the CesA gene family researchers were able to ...
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/small-interfering-rna
Small interfering RNA (siRNA) mediated inhibition of gene expression has rapidly become a major tool for in vitro analysis of protein function. In vivo gene silencing by siRNAs will play an important role for target validation and is the first step towards the development of siRNA-based therapeutics.
https://mcmanuslab.ucsf.edu/node/268
Small interfering RNA (siRNA) is also known as short interfering RNA or silencing RNA. In the literature, synthetic siRNA constructs are generally denoted by "gene name" - siRNA (e.g. p53-siRNA), with constructs targeting the same gene distinguished by the addition of numbers after the construct name (e.g. p53-siRNA-1 and p53-siRNA-2).
https://www.researchgate.net/publication/38039955_Peptide_Mediated_siRNA_Delivery
Peptide Mediated siRNA Delivery. ... In the 1990s an unexpected gene-silencing phenomena in plants, the later called RNA interference (RNAi), perplexed scientists. Following the proof of activity ...
https://www.frontiersin.org/articles/10.3389/fpls.2017.00200/full
Introduction. Mycotoxin contamination in food and feed crops, both pre- and post-harvest by phytopathogenic fungi is a major concern worldwide (Ismaiel and Papenbrock, 2015).Exposure to mycotoxins in humans and livestock occurs mainly through ingestion of contaminated seeds or other edible plant parts.
https://www.sciencedirect.com/science/article/pii/S0168365913009681
Chitosan nanoparticles for siRNA delivery: Optimizing formulation to increase stability and efficiency. ... All of these barriers need to be taken into account to develop powerful siRNA delivery systems. Chitosan is one of the most commonly studied polymers in non-viral gene therapy.
https://en.wikipedia.org/wiki/RNA_interference
RNA interference is a vital part of the immune response to viruses and other foreign genetic material, especially in plants where it may also prevent the self-propagation of transposons. Plants such as Arabidopsis thaliana express multiple dicer homologs that are specialized to react differently when the plant is exposed to different viruses.
http://bmlaser.physics.ecu.edu/literature/2006%2008_Efficient%20delivery%20of%20small%20interfering%20RNA%20to%20plant%20cells%20by%20a%20nanosecond%20pulsed%20laser-induced%20stress%20wave.pdf
Efficient delivery of small interfering RNA to plant cells by a nanosecond pulsed laser-induced stress wave for posttranscriptional gene silencing Wei Tanga,*, Douglas A. Weidnerb, Benjamin Y. Huc, Ronald J. Newtona, Xin-Hua Hud a Department of Biology, Howell Science Complex, East Carolina University, Greenville, NC 27858-4353, USA
https://www.jove.com/video/57390/double-stranded-rna-oral-delivery-methods-to-induce-rna-interference
Clay delivery methods of dsRNA into plants have been shown to provide dsRNA into potted citrus trees, and other plants for over 14 months (data not shown). This delivery system can be used to alter plant traits, such as flower colors, plant height (dwarfing), or others.
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