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https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rnai-sirna/tech-notes/getting-started-with-rnai-in-vivo.html
Because of its potency, siRNA has rapidly become the most widely used trigger for inducing gene knockdown in cultured mammalian cells. siRNA delivery in vivo is a more challenging task. This article describes an RNAi experiment in an animal model in which siRNA was administered using hydrodynamic tail vein injection.
http://altogenlabs.com/rna-interference-rnai-services/in-vivo-sirna-delivery-and-tissue-targeting/
In Vivo siRNA Delivery and Tissue Targeting RNAi-induced gene silencing is used to study gene function in cultured cells ( in vitro ). Most often, these effects are induced by the introduction of small-interfering RNA (siRNA) or microRNA (miRNA).
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995415/
May 05, 2010 · To improve the evaluation of siRNA delivery in vivo, we tailored an approach that quantifies the small RNAs residing in the RISC from the tissues of rodents and monkey. Application of our approach to preclinical animal studies yielded quantitative insights into the in vivo efficiency of siRNA delivery and the mechanism of RNA silencing.Author: Yi Pei, Paula J. Hancock, Hangchun Zhang, René Bartz, Craig Cherrin, Nathalie Innocent, Colin J. Pom...
https://www.sciencedirect.com/science/article/pii/S2211286314000025
However, there are challenges associated with the in vivo delivery of siRNA, such as enzymatic instability and low cellular uptake. In siRNA delivery, non-viral vectors such as cationic liposomes and cationic polymers have been more commonly used than viral vectors.Author: Yoshiyuki Hattori, Ayako Nakamura, Shohei Arai, Mayu Nishigaki, Hiroyuki Ohkura, Kumi Kawano, Yoshie...
https://ashpublications.org/blood/article/113/12/2646/25005/A-novel-in-vivo-siRNA-delivery-system-specifically
Despite growing interest in the application of siRNA into in vivo systems, the most challenging hurdle has been in vivo delivery in general, and specifically the ability to selectively target siRNA to specific cells. 2 Over the past several years, multiple strategies have been developed and assessed in vivo, which can be generally categorized into viral and nonviral methods. 1,3 Despite the high transfection efficiency …Author: Xiufen Zheng, Xiufen Zheng, Costin Vladau, Xusheng Zhang, Xusheng Zhang, Motohiko Suzuki, Thomas E. ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207430/
Small interfering RNAs (siRNAs) have the potential to help study and treat endothelial cells in vivo by durably silencing multiple genes simultaneously, but efficient siRNA delivery …Author: James E. Dahlman, Carmen Barnes, Omar F. Khan, Aude Thiriot, Siddharth Jhunjunwala, Taylor E. Shaw, ...
https://www.sciencedirect.com/science/article/pii/S0168365918300774
In summary, VIPER/siRNA polyplexes efficiently deliver siRNA in vivo resulting in robust gene silencing (>75% knockdown) within the lung. Graphical abstract Download : Download high-res image (134KB)Author: Daniel P. Feldmann, Yilong Cheng, Rima Kandil, Yuran Xie, Mariam Mohammadi, Hartmann Harz, Akhil Sha...
https://advances.sciencemag.org/content/6/13/eaaz6108.full
Small interfering RNA (siRNA) is a powerful tool for gene silencing that has been used for a wide range of biomedical applications, but there are many challenges facing its therapeutic use in vivo....
http://www.invivotransfection.com/
An in vivo transfection reagent that can protect from degradation and deliver the API into cells by endocytosis must also have the ability to release the cargo upon delivery. Efficiency, protecting the API, injection route and lack of immune response all need careful consideration in reagent choice.
https://www.polyplus-transfection.com/products/in-vivo-jetpei-preclinical/
Figure 1. in vivo-jetPEI ® is a safe method of delivery, with no major inflammatory response triggered upon injection. Complexes were formed in 200 µl of 5% glucose using 40 µg of luciferase siRNA with in vivo-jetPEI ® at an N/P ratio of 8, and injected through retro-orbital sinus. 1 to 6 hours after injection, blood was collected and the level of TNF, IFN and IL-6 was measured by ELISA (n=8).
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