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https://www.ncbi.nlm.nih.gov/pubmed/15017078
Adenovirus-delivered siRNA. Shen C(1), Reske SN. Author information: (1)Department of Nuclear Medicine, University Hospital of Ulm, Germany. RNA interference is the process that double-stranded RNA (dsRNA) induces the homology-dependent degradation of cognate mRNA mediated by 21- to 23-nt small interfering RNA (siRNA).Author: Changxian Shen, Sven N. Reske
https://www.thermofisher.com/us/en/home/life-science/rnai/delivering-rnai-to-cells-transfection-and-viral-delivery.html.html
The two common approaches for RNAi delivery are lipid-mediated transfection and viral-mediated transduction.Determining which one of these approaches to use depends on the cell type being studied and whether transient or stable knockdown is desired (Tables 1 & 2).
http://www.bio-medicine.org/biology-technology/Delivering-siRNA-Using-Adenoviral-Vectors-957-1/
Delivering siRNA Using Adenoviral Vectors ... Also, adenovirus gene delivery typically results in higher levels of RNA expression. Infection is independent of cell cycle, so adenoviruses can be used to express RNA in both dividing and non-dividing cells. Integration of the adenoviral DNA into the host genome is rare, which means that there is ...
https://cancerres.aacrjournals.org/content/66/19/9736
Oct 01, 2006 · Despite successes in animal models, cancer gene therapy with small interfering RNAs (siRNA) is hindered by the lack of an optimal delivery platform. We examined the applicability of the replication-competent, oncolytic adenovirus, ONYX-411, to deliver a mutant K-ras siRNA transgene to human cancer cells.Author: Yu-An Zhang, John Nemunaitis, Shirley K. Samuel, Patrick Chen, Yuqiao Shen, Alex W. Tong
https://www.ncbi.nlm.nih.gov/pubmed/12650936
Mar 27, 2003 · RNA interference is the process that double-stranded RNA induces the homology-dependent degradation of cognate mRNA mediated by 21-23 nucleotide short interfering RNA (siRNA). Here, we describe a simple virus vector for efficient delivery of siRNA into mammalian cells utilizing the well-defined H1-RNA promoter and conventional adenovirus.Author: Changxian Shen, Andreas K Buck, Xiangwei Liu, Michael Winkler, Sven N Reske
https://www.sciencedirect.com/science/article/pii/S0014579303002096
The availability of high virus titer, infection of a broad spectrum of cell types and independence on active cell division makes adenovirus the vector of choice for siRNA delivery. The human adenovirus serotype 5 was used in our system.Author: Changxian Shen, Andreas K Buck, Xiangwei Liu, Michael Winkler, Sven N Reske
http://signagen.com/adenovirus-faqs
SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... : Adenovirus FAQs - > In Vitro DNA Transfection Reagents > In Vivo Transfection Reagents > Pre-optimized Transfection Reagents > In Vitro siRNA Transfection Reagents > Transfection Accessories > Pre-made Adenovirus > …
https://www.researchgate.net/publication/10843688_Gene_silencing_by_adenovirus-delivered_siRNA
Gene silencing by adenovirus-delivered siRNA. ... Electroporation-based siRNA delivery is an upcoming therapeutic tool to deliver various siRNAs, both in vitro and in vivo. Discussed in this ...
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