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http://altogenlabs.com/rna-interference-rnai-services/in-vivo-sirna-delivery-and-tissue-targeting/
In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) In Vivo siRNA Delivery and Tissue Targeting. RNAi-induced gene silencing is used to study gene function in cultured cells (in vitro).Most often, these effects are induced by the introduction of small-interfering RNA (siRNA) or microRNA (miRNA).
https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rnai-sirna/tech-notes/getting-started-with-rnai-in-vivo.html
Co-delivery of siRNA with reporter vectors by hydrodynamic tail vein injection provides a starting point for researchers interested in performing RNAi experiments in the liver. There is a need to develop alternative techniques to enable specific delivery to other organs using normal low-pressure injection conditions.
http://www.invivotransfection.com/
In Vivo Transfection In Vivo Background . With modern genetic techniques allowing scientists to identify the link between genes and diseases, there is an enormous emphasis on determining ways to alter the function of a gene in vivo.Strategies to treat these diseases include delivering an alternate gene to the cell, replacing the defective gene or silencing the defective gene using RNAi or ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995415/
May 05, 2010 · In vivo quantification of Ago2-associated siRNA and target gene silencing. To further validate our method and gain quantitative insights into siRNA delivery in vivo, we performed a mouse study measuring both gene silencing and Ago2-associated siRNA strand levels from tissue samples.Author: Yi Pei, Paula J. Hancock, Hangchun Zhang, René Bartz, Craig Cherrin, Nathalie Innocent, Colin J. Pom...
http://altogenlabs.com/rna-interference-rnai-services/
RNA Interference (RNAi) technology has made it possible to effectively regulate gene expression. The application of this technology in the laboratory has given scientists a glimpse into the complex biological systems of humans and mammals alike.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631009/
Jun 13, 2019 · The chemical structure of lipid has a profound effect on the efficacy of siRNA in vivo delivery. It has been reported that the intravenously injected chol-siRNA exist as three forms (complex with LDL, HDL, and albumin), all of which are correlated with different transport pathways and biodistribution profiles .Author: Wanyi Tai
https://www.biosyn.com/tew/In-vivo-delivery-of-lipophilic-siRNA.aspx
In vivo delivery of lipophilic siRNA RNA interference (RNAi) is a conserved biological process for specific silencing of gene expression. Small interfering RNAs ( siRNAs ) are important tools for the control of gene expression, such as post-transcriptional gene silencing in mammals, including humans.
Advirna is developing innovative solutions for oligonucleotide delivery to cells ex-vivo and in-vivo. Our technology platform - self-deliverable RNAi technology (sdRNAi) allows efficient knockdown of genes in hard-to-transfect cell lines ex vivo and in vivo using RNAi interference. sdRNAi technology is based on the deep chemical modification of siRNAs and allows efficient delivery of RNAi into ...
https://www.researchgate.net/publication/336161535_Rekindling_RNAi_Therapy_Materials_Design_Requirements_for_In_Vivo_siRNA_Delivery
Rekindling RNAi Therapy: Materials Design Requirements for In Vivo siRNA Delivery. ... The challenges in in vivo RNAi and materials design requirements to overcome them are reviewed. Recent trends ...
https://altogen.com/transfection-resource/rna-interference-rnai/
In Vivo Transfection Kits from Altogen Biosystems. RNA interference (RNAi) has been used for in vivo target validation studies using animal models. The major challenge in performing RNAi studies in vivo is the effective, directed delivery of functional small RNA molecules into specific tissues.
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