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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Viral and nonviral delivery systems for gene delivery. ... Although numerous viral and nonviral gene delivery systems have been developed in the last 3 decades, ... Retroviral vectors are one of the most frequently employed forms of gene delivery in somatic and germline gene therapies.
https://www.labome.com/method/Nucleic-Acid-Delivery-Lentiviral-and-Retroviral-Vectors.html
Lentiviral and retroviral gene delivery systems exploit aspects of retrovirus replication to provide stable integration of the desired nucleic acid sequence. Whereas transfection of nucleic acids results only in transient transgene expression, the activity of the viral integrase in retroviral and lentiviral-based systems allows for stable ...Author: Erin P O’Keefe
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605697/
Jul 18, 2012 · Retroviral and lentiviral vectors have proven to be particularly efficient systems to deliver genes of interest into target cells, either in vivo or in cell cultures. They have been used for some time for gene therapy and the development of gene vaccines. ...Author: Inès Dufait, Inès Dufait, Therese Liechtenstein, Alessio Lanna, Christopher Bricogne, Roberta Larang...
https://www.pnas.org/content/99/23/14943
Nov 12, 2002 · Retroviral delivery of siRNA into primary fibroblasts eliminates p53 expression. (A) FACS analysis of CD4 expression on primary human fibroblasts infected with LTRH1-p53 or mock-infected. (B) Primary human fibroblasts (AG01522 cells) were treated with 100 μM etoposide for the indicated times, ...Author: Gregory M. Barton, Ruslan Medzhitov
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-2-15
Aug 28, 2002 · Development of a retroviral siRNA delivery vector. Recently, several DNA-based RNAi vectors have become available [4–7].We initially employed the pBS/U6 vector (now sold as pSilencer 1.0-U6, available from Ambion, Austin, TX) which contains the RNA PolIII-specific U6 gene promoter along with restriction sites convenient for oligonucleotide insertion.Author: Eric Devroe, Pamela A Silver
https://www.nature.com/articles/s41375-018-0106-0
Mar 22, 2018 · Lentivirus biology. The basic genes required for retroviral and lentiviral survival and function are the gag, pol, and env genes; gag encodes structural proteins, pol …Author: Michael C. Milone, Una O’Doherty
https://gvt-journal.biomedcentral.com/articles/10.1186/1479-0556-2-9
Aug 13, 2004 · Retroviral vector-mediated gene transfer has been central to the development of gene therapy. Retroviruses have several distinct advantages over other vectors, especially when permanent gene transfer is the preferred outcome. ... A review of retroviral pathogenesis and its relevance to retroviral vector-mediated gene delivery. Donald S Anson 1 ...Author: Donald S Anson, Donald S Anson, Donald S Anson
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