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https://www.researchgate.net/publication/11047689_Retroviral_delivery_of_small_interfering_RNA_into_primary_cell
Retroviral delivery of small interfering RNA into primary cell Article in Proceedings of the National Academy of Sciences 99(23):14943-5 · December 2002 with 8 Reads How we measure 'reads'
https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(16)31810-X
RNA interference (RNAi) has tremendous potential for investigating gene function and developing new therapies. However, the design and validation of proficient vehicles for stable and safe microRNA (miR) and small interfering RNA (siRNA) delivery into relevant target cells …
https://cancerres.aacrjournals.org/content/65/7/2705
Down-regulation of Cx43 by Retroviral Delivery of Small Interfering RNA Promotes an Aggressive Breast Cancer Cell Phenotype. ... Engineering of the Cx43 small interfering RNA constructs and retroviral short hairpin RNA vector. ... Medzhitov R. Retroviral delivery of small interfering RNA into primary cells.
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-2-15
Retrovirus-delivered siRNA provides significant advancement over previously available methods by providing efficient, uniform delivery and immediate selection of stable "knock-down" cells. This development should provide a method to rapidly assess gene function in established cell lines, primary cells, or animals.
http://www.nature.com/articles/1206733
Aug 28, 2003 · Post-transcriptional gene silencing by small interfering RNAs (siRNAs) is rapidly becoming a powerful tool for genetic analysis of mammalian cells. Delivery of siRNA into mammalian cells …
https://www.genscript.com/tech_guide/tm0176.pdf
delivery and achieved stable long-term expression of the transgenes. ... To deliver the siRNA expression system into mammalian cells using GenScript Retroviral vectors, you also need ... Barton GM, and Medzhitov R. (2002) Retroviral delivery of small interfering RNA into primary cells. Proc Natl Acad Sci U S A 99(23):14943-14945. 8.
https://www.researchgate.net/publication/254263945_Infection_of_Mammalian_Cells_with_Retroviral_shRNAs
Infection of Mammalian Cells with Retroviral shRNAs. ... has the potential to immortalize primary B cells into lymphoblastoid cell lines (LCLs) in vitro. ... Retroviral delivery of small ...
https://www.cell.com/molecular-therapy-family/molecular-therapy/pdf/S1525-0016(16)31810-X.pdf
RNA interference (RNAi) has tremendous potential for investigating gene function and developing new thera-pies. However, the design and validation of proficient vehicles for stable and safe microRNA (miR) and small interfering RNA (siRNA) delivery into relevant target cells remains an active area of investigation. Here, we devel-
https://www.sciencedirect.com/science/article/pii/S1525001606001808
This work introduces a novel chitosan-based siRNA nanoparticle delivery system for RNA interference in vitro and in vivo. The formation of interpolyelectrolyte complexes between siRNA duplexes (21-mers) and chitosan polymer into nanoparticles, ranging from 40 to 600 nm, was shown using atomic force microscopy and photon correlation spectroscopy.
https://www.nature.com/articles/3302878
The use of a herpes simplex virus type 1 (HSV-1)-based amplicon vector for siRNA delivery into mammalian cells, using human polyomavirus BK (BKV)-transformed cells …
https://www.jci.org/articles/view/JCI20673
RNAi is an evolutionarily conserved process of posttranscriptional suppression of gene expression and is mediated by 21- to 23-nucleotide (nt) sequence-specific double-stranded small interfering RNA (siRNA) in mammalian cells without initiating the nonspecific double-stranded RNA responses (10 – 12).
https://www.sciencedirect.com/science/article/pii/S0301562913010661
Small interfering RNA was successfully delivered into 97.9% of all cells, and resulted in 70% down-regulation of the GAPDH protein, whereas Cy3-labeled pDNA encoding eGFP was delivered into 43% of the cells, of which only 2% exhibited eGFP expression. Furthermore, transfection with pDNA encoding GAPDH failed to increase cellular GAPDH levels.
https://arthritis-research.biomedcentral.com/articles/10.1186/ar1168
Mar 10, 2004 · Despite its potential, the major obstacle to the use of RNAi (as for all previous gene silencing approaches) is the need for efficient and sustained delivery of small interfering RNA into primary mammalian cells, and specific targeting of particular cell types in vivo.
https://link.springer.com/protocol/10.1007%2F978-1-59745-237-3_18
These properties make lentiviral vectors attractive vehicles for delivering small interfering RNA (siRNA) genes into mammalian cells. RNA polymerase III (Pol III) promoters are most commonly used for expressing siRNAs from lentiviral vectors. Pol III promoters are relatively small, have high activity, and use simple termination signals of short ...
https://onlinelibrary.wiley.com/doi/full/10.1111/j.1600-6143.2004.00530.x
To take advantage of the gene‐silencing effect while circumventing nonspecific cellular effects, synthetic dsRNA of 21 nucleotides are transfected into the cell. This small interfering RNA (siRNA) is not recognized by DICER or 2′5 oligosynthetase, but instead directly binds the RISC complex that subsequently induces selective silencing of ...
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