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https://www.ncbi.nlm.nih.gov/pubmed/29151352
Dec 20, 2017 · Plasmid DNA molecules with unique loop structures have widespread bioapplications, in many cases relying heavily on delivery vehicles to introduce them into cells and achieve their functions. Herein, we demonstrate that control over delicate nanotopography of silica nanoparticles as plasmid DNA vectors has significant impact on the transfection ...Author: Hao Song, Meihua Yu, Yao Lu, Zhengying Gu, Yannan Yang, Min Zhang, Jianye Fu, Chengzhong Yu
https://pubs.acs.org/doi/10.1021/jacs.7b08974
Plasmid DNA molecules with unique loop structures have widespread bioapplications, in many cases relying heavily on delivery vehicles to introduce them into cells and achieve their functions. Herein, we demonstrate that control over delicate nanotopography of silica nanoparticles as plasmid DNA vectors has significant impact on the transfection efficacy.Author: Hao Song, Meihua Yu, Yao Lu, Zhengying Gu, Yannan Yang, Min Zhang, Jianye Fu, Chengzhong Yu
https://www.sciencedirect.com/science/article/pii/S0168365907006220
Biodegradable polymers as non-viral carriers for plasmid DNA delivery. ... Also, the degradation of the polymer can be used as a tool to release the plasmid DNA into the cytosol. In this article the recent results obtained with two classes of degradable gene delivery systems, namely those based on water-soluble cationic polymers and on micro ...Author: Jordy Luten, Cornelus F. van Nostrum, Stefaan C. De Smedt, Wim E. Hennink
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109217/
Although the exact mechanism of translocation of DNA across the membrane pores is debated (electrophoretic facilitation versus passive diffusion) the end result of the process is that upwards of 100–1000 fold enhancement of plasmid delivery and gene expression can be achieved relative to delivery of DNA alone without electroporation (see ...Author: Niranjan Y Sardesai, David B Weiner
https://www.sciencedirect.com/science/article/pii/S0169409X15002392
Co-delivery of anti-cancer drugs and plasmid DNA using separate carriers. As discussed earlier, cancer is a difficult disease to treat due to its heterogeneous disease manifestation as well as pathogenic pathways. This necessitates tailored and sophisticated therapeutic modalities for effective treatment. Polymer-based chemotherapeutic drug or ...Author: Pei Yun Teo, Wei Cheng, James L. Hedrick, Yi Yan Yang
https://www.precisionnanosystems.com/resource-center/publications/detail/plasmid-lnp-kulkarni2016
Dec 28, 2016 · With siRNA being a very different nucleic acid species from plasmids, the LNP systems require optimization for effective encapsulation and delivery of plasmid DNA. In this article, the Cullis lab optimized LNP systems for transfection by modifying lipid composition and plasmid-to-lipid ratio.
https://www.mirusbio.com/products/accessories/label-it-plasmid-delivery-controls
Label IT® Cy®3 Plasmid Delivery Control Allows Quick Assessment of Delivery Efficiency. HeLa cells were transfected in serum-containing media with the Label IT® Cy®3 Plasmid Delivery Control (red) using the TransIT®-LT1 Transfection Reagent. Twenty-four hours post-transfection, the cells were fixed, then counterstained to locate the nuclei ...
https://www.pnas.org/content/99/23/14640
Nov 12, 2002 · The effect of various chemical modifications of nitrogen atoms on the efficiency of polyethylenimines (PEIs) as synthetic vectors for the delivery of plasmid DNA into monkey kidney cells in vitro has been systematically investigated. The resultant structure–activity relationship has both provided mechanistic insights and led to PEI derivatives with markedly enhanced performance. For example ...Author: Mini Thomas, Alexander M. Klibanov
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