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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722627/
Alternative gene therapy . Another means of using bacteria for gene therapy is the so-called Alternative gene therapy (AGT) approach, which is also known as bacterial protein delivery. It is based on the transfer of bacterially expressed therapeutic proteins to the host organism using genetically modified (transformed) bacteria.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · INTRODUCTION. Basically gene therapy is an intracellular delivery of genomic materials (transgene) into specific cells to generate a therapeutic effect by correcting an existing abnormality or providing the cells with a new function.[] Different types of gene delivery systems may be applied in gene therapy to restore a specific gene function or turning off a special gene(s).
https://www.nature.com/articles/3301308
Nov 06, 2000 · the structure of ‘stabilized plasmid-lipid particles’ (splp) and their properties as systemic gene therapy vectors has been investigated. we show that splp can be visualized employing cryo ...Author: P. Tam, M. Monck, D. Lee, O. Ludkovski, E. C. Leng, K. Clow, H. Stark, P. Scherrer, R. W. Graham, P....
https://www.nature.com/articles/3301173
May 25, 2000 · Gene therapy by direct delivery of plasmid DNA has several advantages over viral gene transfer, but plasmid delivery is less efficient. In vivo electroporation has been used to …Author: L. Heller, M. J. Jaroszeski, D. Coppola, C. Pottinger, R. Gilbert, Richard Heller
https://www.sciencedirect.com/science/article/pii/S0168365907006220
Gene therapy holds a great promise for the treatment of acquired and inherited diseases with a genetic origin that are currently incurable. Non-viral gene delivery systems are gaining recognition as an alternative to viral gene vectors for their potential in avoiding immunogenicity and toxicity problems inherently associated with the use of viral systems.Author: Jordy Luten, Cornelus F. van Nostrum, Stefaan C. De Smedt, Wim E. Hennink
https://www.researchgate.net/publication/23656738_Upstream_Processing_of_Plasmid_DNA_for_Vaccine_and_Gene_Therapy_Applications
The demand for plasmid DNA has increased vastly in response to rapid advances in its use in gene therapy and vaccines. These therapies are based on the same principle, i.e. the introduction of ...
http://www.biologydiscussion.com/gene/therapy/gene-therapy-ex-vivo-and-in-vivo-gene-therapy-with-diagram/9969
II. In vivo gene therapy: The direct delivery of genes into the cells of a particular tissue is referred to as in vivo gene therapy. Type # I. Ex Vivo Gene Therapy: The ex vivo gene therapy can be applied to only selected tissues (e.g., bone marrow) whose cells can be cultured in the laboratory.
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