Plasmid Delivery In Vivo

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Label IT Plasmid Delivery Controls - Research Reagent Products

    https://www.mirusbio.com/products/accessories/label-it-plasmid-delivery-controls
    Label IT® Cy®3 Plasmid Delivery Control Allows Quick Assessment of Delivery Efficiency. HeLa cells were transfected in serum-containing media with the Label IT® Cy®3 Plasmid Delivery Control (red) using the TransIT®-LT1 Transfection Reagent. Twenty-four hours post-transfection, the cells were fixed, then counterstained to locate the nuclei ...

519. In Vivo Delivery of Nucleic Acid to the Brain Using ...

    https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(16)38877-3
    May 01, 2009 · Using an in vivo imaging system (IVIS), we assessed the level of luciferase gene activity on days 3 and 7 post-delivery of the DNA nanoparticles. Following IVIS collection on day 7, brain tissue lysates were prepared and used to further assess luciferase expression with …

Plasma-activated air mediates plasmid DNA delivery in vivo

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830379/
    Plasma-activated air (PAA) provides a noncontact DNA transfer platform. In the current study, PAA was used for the delivery of plasmid DNA in a 3D human skin model, as well as in vivo.Delivery of plasmid DNA encoding luciferase to recellularized dermal constructs was enhanced, resulting in a fourfold increase in luciferase expression over 120 hours compared to injection only (P < 0.05).Author: Chelsea M Edelblute, Loree C Heller, Muhammad A Malik, Anna Bulysheva, Richard Heller

Delivery Approaches for CRISPR/Cas9 Therapeutics In Vivo ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295289/
    Areas Covered. In this topical review, we highlight recent advances to in vivo delivery of the CRISPR/Cas9 system using various packaging formats, including viral, mRNA, plasmid, and protein-based approaches. We also discuss some of the barriers which have yet to be overcome for successful translation of this technology.Author: D.C. Luther, Y.W. Lee, H. Nagaraj, F. Scaletti, V.M. Rotello

Design of Lipid nanoparticles for in Vitro and in Vivo ...

    https://www.precisionnanosystems.com/resource-center/publications/detail/plasmid-lnp-kulkarni2016
    Dec 28, 2016 · In this article, the Cullis lab optimized LNP systems for transfection by modifying lipid composition and plasmid-to-lipid ratio. The authors used the NanoAssemblr TM Benchtop to formulate a plasmid sequence into an LNP carrier and demonstrated highly efficient uptake and exogenous gene expression in vitro and in vivo.

Design of lipid nanoparticles for in vitro and in vivo ...

    https://www.sciencedirect.com/science/article/pii/S1549963416302313
    Lipid nanoparticles (LNPs) containing distearoylphosphatidlycholine (DSPC), and ionizable amino-lipids such as dilinoleylmethyl-4-dimethylaminobutyrate (DLin-MC3-DMA) are potent siRNA delivery vehicles in vivo.Here we explore the utility of similar LNP systems as transfection reagents for plasmid DNA (pDNA).Author: Jayesh A. Kulkarni, Johnathan Layne Myhre, Sam Chen, Yuen Yi C. Tam, Adrian Danescu, Joy M. Richman,...

Electroporation Method for In Vivo Delivery of Plasmid DNA ...

    https://www.jove.com/video/60066/electroporation-method-for-vivo-delivery-plasmid-dna-adult-zebrafish
    Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon. Tamara Durovic 1,2, Jovica Ninkovic 1,3,4. 1 Institute of Stem Cell Research, Helmholtz Zentrum München, ...Author: Tamara Durovic, Jovica Ninkovic

Plasmid Delivery in Vivo from Porous Tissue-Engineering ...

    https://www.sciencedirect.com/science/article/pii/S1525001605001814
    Plasmid Delivery in Vivo from Porous Tissue-Engineering Scaffolds: Transgene Expression and Cellular Transfection. ... Plasmid delivery from natural and synthetic polymers have allowed transgene expression for weeks to months 11., 34., 35.. At low plasmid loadings, the initial burst may transfect cells for the short term, but an insufficient ...Author: Jae Hyung Jang, Christopher B. Rives, Lonnie D. Shea

Effective in vivo gene delivery with reduced toxicity ...

    https://www.nature.com/articles/s41598-017-17316-y
    Dec 06, 2017 · Even more curious, PF6 has been shown to be an excellent siRNA delivery vector in vivo 11, suggesting that different delivery bottlenecks exist …Author: Kaido Kurrikoff, Kadi-Liis Veiman, Kadri Künnapuu, Elin Madli Peets, Tõnis Lehto, Ly Pärnaste, Piret...

TurboFect in vivo Transfection Reagent

    https://www.thermofisher.com/order/catalog/product/R0541
    Thermo Scientific TurboFect in vivo Transfection Reagent is highly effective for DNA plasmid delivery in vivo, while not inducing an inflammatory response. TurboFect in vivo has been assessed for plasmid DNA delivery in both mice and rats and is suitable for DNA administration via various routes, in

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