Plasmid Delivery Filter Paper

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Shipping and Receiving Plasmids on Filter Paper

    http://wang.ucsd.edu/protocol/2.%20molecular%20cloning/2.3%20Amplification/Shipping_and_Receiving_Plasmids_on_Filter_Paper.pdf
    Shipping and Receiving Plasmids on Filter Paper Sending: 1) Mark a circled area with a pencil (not a marker pen) on a clean Whatman #1 filter paper (or equivalent). 2) Spot about 2 µg of plasmid DNA into the circle. Allow the filter paper dry at room temperature. 3) Insert spotted filter paper inside a …File Size: 21KB

Shipping of Plasmids on Filter Paper - MCDB

    https://labs.mcdb.ucsb.edu/weimbs/thomas/sites/labs.mcdb.ucsb.edu.weimbs.thomas/files/docs/shipping_of_plasmids_on_filter_paper.pdf
    Shipping of Plasmids on Filter Paper Sending: 1) Mark a circled area with a pencil (not a marker pen) on a clean Whatman #1 filter paper (or equivalent). 2) Spot about 2 µg of plasmid DNA into the circle. Allow the filter paper dry at room temperature.File Size: 233KB

How do I process my Addgene plasmid (DNA tube/filter paper ...

    https://help.addgene.org/hc/en-us/articles/206127485-How-do-I-process-my-Addgene-plasmid-DNA-tube-filter-paper-
    How do I process my Addgene plasmid (DNA tube/filter paper)? Some countries can only receive plasmids in the form of DNA on filter paper or in a tube. DNA on filter paper. If you are receiving plasmids in this format, Addgene provides you with two spots of …

Sending Plasmids: How to Avoid Jail Time ... - Bitesize Bio

    https://bitesizebio.com/394/sending-plasmids-how-to-avoid-jail-time-and-shredded-envelopes/
    On filter paper. Another way to get your plasmid through the automatic sorting machines is to spot it onto a very small disc of filter paper. Cut out the filter paper, spot 10 microlitres of plasmid solution onto it, allow it to dry for a few minutes, then cover with sandwich wrap.

extraction of plasmid DNA from the paper Scientist Solutions

    http://scientistsolutions.com/forum/cloning-recombinant-gene-expression-plasmidscosmidsbacpac/extraction-plasmid-dna-paper/
    Apr 29, 2009 · I received a plasmid that I requested from an overseas lab and they shipped it to me on a piece of filter paper. There is small amount of plasmid spotted on the paper. Does anyone have any suggestions on how to remove such a small amount of plasmid and recover enough that I can carry on with a transformation? ... Cut out the area of filter ...

How can I elute plasmid dried on paper and transform ...

    https://www.researchgate.net/post/How_can_I_elute_plasmid_dried_on_paper_and_transform_chemically_competent_E_coli_cells_with_it
    How can I elute plasmid dried on paper and transform chemically competent E coli cells with it? ... Do you know the amount of plasmid spotted on the filter paper in order to have an idea about the ...

How can I elute plasmid from filter paper?

    https://www.researchgate.net/post/How_can_I_elute_plasmid_from_filter_paper
    I received a plasmid that I requested from another lab and they shipped it to me on a filter paper. Give me any suggestions and protocol on how to remove and transform plasmid into E.coli, and my ...

Addgene: You have just received your plasmids from Addgene ...

    https://www.addgene.org/recipient-instructions/myplasmid/
    DNA - Filter Paper or Tube DNA on Filter Paper. Your plasmids have been provided in the form of DNA on filter paper. Addgene provides you with two spots of DNA for each plasmid. It can be stored at 4°C. To recover your plasmid: To recover the plasmid, use a clean razor blade to cut out one of the circles containing your DNA.

Team:Cambridge/Protocols/Filter Paper - 2011.igem.org

    http://2011.igem.org/Team:Cambridge/Protocols/Filter_Paper
    Spotting DNA on filter paper is an easy and safe way of plasmid shipment widely used in the scientific world. Negatively charged DNA is firmly bound to cellulose fibers of the blotting paper, and remains stable at room temperature for several days, if contamination is avoided. Practice DNA spotting on filter paper. Spot 2 μL of DNA solution on ...

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