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https://www.ncbi.nlm.nih.gov/pubmed/29151352
Dec 20, 2017 · Plasmid DNA molecules with unique loop structures have widespread bioapplications, in many cases relying heavily on delivery vehicles to introduce them into cells and achieve their functions. Herein, we demonstrate that control over delicate nanotopography of silica nanoparticles as plasmid DNA vectors has significant impact on the transfection ...Author: Hao Song, Meihua Yu, Yao Lu, Zhengying Gu, Yannan Yang, Min Zhang, Jianye Fu, Chengzhong Yu
https://www.mirusbio.com/products/accessories/label-it-plasmid-delivery-controls
Visualization of Label IT® Fluorescein Plasmid Delivery Control in Liver Sections Following Tail Vein Injection. TransIT®-QR Hydrodynamic Delivery Solution was used to deliver 25 µg of Label IT® Fluorescein Plasmid Control (green) to a mouse using hydrodynamic delivery via the tail vein. One hour post-injection the liver was harvested. Sections were fixed to stain the nuclei (blue) and the ...
https://onlinelibrary.wiley.com/doi/abs/10.1002/marc.201800068
Whereas PC can condense and encapsulate large plasmids at high N/P ratio, the delivery of plasmid results in efficient editing at two genome loci, namely, hemoglobin subunit beta (19.1%) and rhomboid 5 homolog 1 (RHBDF1) (7.0%). Sanger sequencing further …Author: Zhen Zhang, Tao Wan, Tao Wan, Yuxuan Chen, Yu Chen, Hongwei Sun, Tianqi Cao, Zhou Songyang, Guping T...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006671/
Kenny et al. developed an MRI-visible gene delivery nanocomplex system comprised of self-assembling mixtures of liposomes, plasmid DNA, and targeting ligands, which specifically enhanced transfection efficiency and allowed real-time in vivo monitoring of the specific tumor tissue (Kenny et al., 2012).Author: Yi Zhao, Leaf Huang
https://pubs.acs.org/doi/abs/10.1021/jacs.7b08974
Plasmid DNA molecules with unique loop structures have widespread bioapplications, in many cases relying heavily on delivery vehicles to introduce them into cells and achieve their functions. Herein, we demonstrate that control over delicate nanotopography of silica nanoparticles as plasmid DNA vectors has significant impact on the transfection efficacy.Author: Hao Song, Meihua Yu, Yao Lu, Zhengying Gu, Yannan Yang, Min Zhang, Jianye Fu, Chengzhong Yu
https://www.sciencedirect.com/science/article/pii/S0168365907006220
1. Introduction. The success of gene therapy largely depends on the availability of suitable delivery vehicles. Although viral vectors display rather good transfection properties, both in vitro and in vivo, there is a large number of problems associated with the use of these vectors , , .These drawbacks include the induction of an immune response against the viral proteins, possible ...Author: Jordy Luten, Cornelus F. van Nostrum, Stefaan C. De Smedt, Wim E. Hennink
https://www.sciencedirect.com/science/article/pii/S0169409X15002392
2.2. Delivery of plasmid DNA using polymeric carriers. Gene delivery vectors could be generally divided into two major categories: viral and non-viral. Despite the high efficacy of viral vectors at delivering genes, their wide spread clinical adoption was often impeded by their tendency to cause immunogenicity and insertional oncogenesis , .Author: Pei Yun Teo, Wei Cheng, James L. Hedrick, Yi Yan Yang
The plasmid ninjas have been perfecting DNA production since 1998. Along with some basic information they only need ≈1µg of starter material and as little as 72 hours to complete production of your ready-to-use plasmid DNA. Talk to a plasmid ninja today: +1 (701) 499-7312. Have special offers delivered direct to your inbox with our ninja emails
https://www.precisionnanosystems.com/resource-center/publications/detail/plasmid-lnp-kulkarni2016
Dec 28, 2016 · To optimize the LNP system for plasmid delivery, Cullis lab modified the well-established siRNA-LNP system by replacing the helper lipid – a saturated phosphatidylcholine (PC) - with unsaturated varieties that are thought to promote structures that enhance membrane fusion and intracellular delivery.
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