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https://www.sciencedirect.com/science/article/pii/S0960894X16312562
Cell-penetrating peptides (CPPs) are oligopeptides that can permeate the cell membrane. The use of a CPP-mediated transport system could be an excellent method for delivering cell-impermeable substances such as proteins, antibodies, antisense oligonucleotides, siRNAs, plasmids, drugs, fluorescent compounds, and nanoparticles as covalently or noncovalently conjugated cargo into cells.Author: Toshihiko Tashima
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222116/
Nov 02, 2018 · Among the numerous limitations, efficient delivery of the CRISPR-Cas9 system into the nuclei of cells without being entrapped in the endosome may be one of the most difficult challenges. Physical delivery approaches including microinjection, electroporation, and hydrodynamic injection have been shown to be efficient for transfection.Author: Xingang Guan, Xingang Guan, Zhimin Luo, Zhimin Luo, Wujin Sun
https://www.nature.com/articles/nbt1201-1173
Dec 01, 2001 · A peptide carrier for the delivery of biologically active proteins into mammalian cells Skip to main content Thank you for visiting nature.com.Author: May C. Morris, Julien Depollier, Jean Mery, Frederic Heitz, Gilles Divita
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3574231/
Feb 15, 2013 · Here, we report a potentially general approach for intracellular delivery of cyclic peptides. Short peptide motifs rich in arginine and hydrophobic residues (e.g., FΦRRRR, where Φ is L-2-naphthylalanine), when embedded into small- to medium-sized cyclic peptides (7–13 amino acids), bound to the plasma membrane of mammalian cultured cells ...Author: Ziqing Qian, Tao Liu, Yu Yu Liu, Roger Briesewitz, Amy M. Barrios, Sissy M. Jhiang, Dehua Pei
https://www.nature.com/articles/s41598-019-55091-0
Dec 10, 2019 · Intracellular delivery of therapeutic antibodies into specific cells using antibody-peptide fusions. ... A peptide carrier for the delivery of biologically active proteins into mammalian cells. ...Author: Julie Gaston, Nicolas Maestrali, Guilhem Lalle, Marie Gagnaire, Alessandro Masiero, Bruno Dumas, Tar...
https://www.jbc.org/content/293/44/17306.full
Abstract. The CRISPR-Cas9 system has developed into a powerful platform for genome editing in various types of cells and tissues with single-nucleotide precision, but limited delivery options hamper its application in real-world settings.Author: Xingang Guan, Xingang Guan, Zhimin Luo, Zhimin Luo, Wujin Sun
https://www.sciencedirect.com/science/article/pii/S0163725815001412
These cell-penetrating peptides, as they are now called, have been shown to not only be capable of crossing the cell membrane themselves but can also carry many different therapeutic agents into cells, including small molecules, plasmid DNA, siRNA, therapeutic proteins, viruses, imaging agents, and other various nanoparticles.Author: Joshua D. Ramsey, Nicholas H. Flynn
https://onlinelibrary.wiley.com/doi/abs/10.1111/cbdd.13537
As expected, histidine‐rich LH could be activated and penetrate into cells at pH 6.0, whereas its membrane transduction activity could be shielded at pH 7.4. In contrast, LK showed no obviously different cellular uptake at both pH conditions.Author: Yun Zhang, Li Li, Linlin Chang, Hui Liu, Jingjing Song, Yue Liu, Hexin Bao, Beijun Liu, Rui Wang, Ji...
https://www.fasebj.org/doi/10.1096/fj.02-0280fje
Dec 18, 2002 · To determine whether the cancer cell binding peptide LTVSPWY could be used to enhance the delivery of antisense oligonucleotides, a fluorescein-conjugated antisense oligonucleotide against the ErbB2 receptor was designed and coupled via a disulfide bridge to the LTVSPWY peptide. SKBR3 cells incubated with the antisense-peptide conjugates for 4 ...Author: Mohsen Shadidi, Mouldy Sioud
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