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https://www.jove.com/video/57390/double-stranded-rna-oral-delivery-methods-to-induce-rna-interference
Most effective RNAi experiments in hemipteran have utilized injection of dsRNA 40 because oral delivery of dsRNA in hemipteran insects is arduous since it must be …Author: Saikat Kumar B. Ghosh, Wayne B. Hunter, Alexis L. Park, Dawn E. Gundersen-Rindal
https://www.academia.edu/25703644/Oral_delivery_of_double-stranded_RNA_in_larvae_of_the_yellow_fever_mosquito_Aedes_aegypti_Implications_for_pest_mosquito_control
RNA interference has already proven itself to be a highly versatile molecular biology tool for understanding gene function in a limited number of insect species, but its widespread use in other species will be dependent on the development of easier
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171847
For sap-sucking insects, such as sharpshooters, aphids, whiteflies and psyllids, dsRNA delivery methods have been demonstrated [29,42,49,50]. In the present study, we demonstrated the induction of specific RNAi effects in ACP by oral acquisition of dsRNA from artificial diets and M. paniculata leaves.
https://www.aquaculturealliance.org/advocate/studies-seek-oral-delivery-of-rnai-based-therapeutics-for-shrimp/
Oral delivery of RNAi therapeutics. Unfortunately, the delivery of potential RNAi-based therapeutics via injection is impractical. Oral delivery approaches are likely to be a more viable option in shrimp aquaculture. To date, simple methods for the oral delivery of dsRNA …
https://www.researchgate.net/publication/255976198_Delivery_of_dsRNA_for_RNAi_in_insects_An_overview_and_future_directions
Delivery of dsRNA for RNAi in insects: An overview and future directions. ... Feeding, also known as oral delivery, involves synthesizing dsRNA in vitro or expressing dsRNA in bacteria, and then ...
https://www.tandfonline.com/doi/pdf/10.1080/19420889.2016.1236163
ARTICLE ADDENDUM Oral delivery of dsRNA by microbes: Beyond pest control Antoine Abrieux and Joanna C. Chiu Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, USA
https://www.nature.com/articles/s41598-017-15534-y
Oral delivery of dsRNA to D. radicum. Preliminary experiments established that larvae would survive when immersed in water for more than 18 hours and that red food dye included in the dipping ...
https://www.sciencedirect.com/science/article/pii/S122686151630259X
RNAi through oral delivery of dsRNA is elucidated in P. xylostella. • JHEH and EcR were chosen as target genes for silencing. • Target gene silencing resulted in mortality which is reliant on dsRNA concentration. • JHEH and EcR proved to be good target genes in bringing out pest management.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0183054
Second, limiting factor of dsRNA was determined through oral administration. Third, to prevent dsRNA degradation and supply large amounts of dsRNA, a recombinant bacterial expression system was used to produce dsRNA. Fourth, bacterial delivery system was modified to facilitate dsRNA release from recombinant bacteria.
https://www.jove.com/video/57385/practical-use-rna-interference-oral-delivery-double-stranded-rna
The oral delivery of dsRNA is particularly interesting for the application of RNAi in a pest control strategy, but a method to retard the degradation of dsRNA by the nucleases in the midgut has not yet been developed, which would have the potential to ensure effective RNAi through feeding.
https://www.sciencedirect.com/science/article/pii/S0168365918300208
Delivery is in the form of dsRNA/BAPC complexes. The biophysical properties of the dsRNA/BAPC complexes are very similar to the BAPCs-DNA complexes previously reported . BAPCs mixed with dsRNA form compact clusters with sizes ranging predominantly from 70 to 500 nm and with zeta potentials ranging from 10 to 18 mV.
https://www.frontiersin.org/articles/10.3389/fphys.2018.01912/full
Jan 11, 2019 · This method of oral dsRNA delivery appeared to be especially effective in the African malaria mosquito A. gambiae, and the yellow fever mosquito A. aegypti. In these two species successful application of chitosan-mediated dsRNA delivery led to …
https://igtrcn.org/tag/dsrna/
The efficacy of RNAi in insects depends in part on efficient delivery and uptake of dsRNA. Gillet et al. (2017) report in Frontiers of Physiology on their use of protein-complexed dsRNA to silence genes in Anthonomous grandis following oral delivery, […] Read More »
https://bmcbiotechnol.biomedcentral.com/track/pdf/10.1186/s12896-017-0328-7
We showed that oral delivery of bacterially expressed dsRNA would lead to RNAi effects in the recipient insect. Quantitative real-time PCR results showed that expression level of target MseChi1 and MseChi2 genes in gut tissue of M. separata were down-regulated after oral delivery of engineered bacteria expressing the corresponding dsRNA.
https://www.researchgate.net/publication/309678257_Oral_delivery_of_dsRNA_by_microbes_Beyond_pest_control_article_addendum
RNA interference (RNAi) by oral delivery of dsRNA in insects has great potential as a tool for integrated pest management (IPM), especially with respect to addressing the need to reduce off-target ...
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