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https://www.ncbi.nlm.nih.gov/pubmed/17555366
Non-viral gene delivery: from the needle to the nucleus. Rettig GR(1), Rice KG. Author information: (1)Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242, USA.Author: Garrett R Rettig, Kevin G Rice
https://www.researchgate.net/publication/6282192_Non-viral_gene_delivery_From_the_needle_to_the_nucleus
Non-viral gene delivery approaches, including those utilizing polycations such as polyethylenimine (PEI) [11], are commonly utilized due, in part, to the safety concerns associated with viral ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782077/
Oct 16, 2009 · The nonviral gene delivery methods, on the other hand, use synthetic or natural compounds or physical forces to deliver a piece of DNA into a cell. The materials used are generally less toxic and immunogenic than the viral counterparts.Author: Mohammed S. Al-Dosari, Xiang Gao
http://cshprotocols.cshlp.org/content/2011/3/top101.full
This article provides an overview of several of these nonviral gene delivery methods, with an emphasis on direct injection of plasmids into cells. ... microinjection of the DNA into the nucleus is strongly recommended. ... The needle is positioned over the site to be injected, and when the ...Author: David A. Dean, Joshua Z. Gasiorowski
https://www.nature.com/articles/3302534
Apr 28, 2005 · Nonviral gene delivery is limited to a large extent by multiple extracellular and intracellular barriers. One of the major barriers, especially in nondividing cells, is the nuclear envelope…Author: D A Dean, D D Strong, W E Zimmer
https://pubs.acs.org/doi/10.1021/bc034140k
Dec 13, 2003 · Nonviral gene delivery is limited by inefficient transfer of DNA from the cytoplasm to the nucleus. Nuclear localization sequence (NLS) peptides have been widely used to exploit intracellular transport mechanisms and promote nuclear uptake of DNA. However, the exact conditions to successfully utilize the properties of NLS peptides are still unclear.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Physical methods of nonviral gene delivery. Physical methods applied for in vitro and in vivo gene delivery are based on making transient penetration in cell membrane by mechanical, electrical, ultrasonic, hydrodynamic, or laser-based energy so …
https://www.sciencedirect.com/science/article/pii/S0168365915302649
Non-viral site-specific gene therapy via phage ФC31-derived integrase system: The phage ФC31 integrase-mediated site-specific integration technology is applied as a DNA vector system for non-viral delivery and insertion of therapeutic gene of interest (GOI) from a donor DNA vector into the specific sites distributed in the recipient cell genome.Author: Marianna Foldvari, Ding Wen Chen, Nafiseh Nafissi, Daniella Calderon, Lokesh Narsineni, Amirreza Raf...
https://www.intechopen.com/books/gene-therapy-tools-and-potential-applications/non-viral-delivery-systems-in-gene-therapy
Gene delivery systems include viral vectors and non-viral vectors. Viral vectors are the most effective, but their application is limited by their immunogenicity, oncogenicity and the small size of the DNA they can transport. Non-viral vectors are safer, of low cost, more reproducible and …Author: Alicia Rodríguez Gascón, Ana del Pozo-Rodríguez, María Ángeles Solinís
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4347098/
Jan 01, 2015 · The major advantage of using non-viral vectors is its bio-safety. However the application of non-viral gene transfer have been ignored for a long time in past because of their poor efficiency of delivery thereby low transient expression of their transgenes . Non-viral vectors have drawn significant attention due to its less immunotoxicity.Author: Murali Ramamoorth, Aparna Narvekar
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