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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Physical methods of nonviral gene delivery. Physical methods applied for in vitro and in vivo gene delivery are based on making transient penetration in cell membrane by mechanical, electrical, ultrasonic, hydrodynamic, or laser-based energy so that DNA entrance into the targeted cells is facilitated.
https://www.ncbi.nlm.nih.gov/pubmed/23437936
Non-viral approaches show high potential due to advantages of relative safety, ability to transfer large size gene, less toxicity and easiness for preparation etc. However, the clinical application of non-viral methods is still restricted by some limitations including low …Author: Weiwei Wang, Wenzhong Li, Nan Ma, Gustav Steinhoff
http://www.eurekaselect.com/106191/article
In order to improve gene transfer efficacy, a lot of efforts have been made in the past years, and numerous gene carriers and techniques have been developed. In this review, we summarized the features, drawbacks and prospects of existing and emerging non-viral gene delivery methods.
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