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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009470/
In vivo miRNA delivery strategies for cancer therapy. A lot of work has been done in exploiting and evaluating the features of tumor and tumor microenvironment to improve RNA delivery. There are two classes of strategies for miRNA delivery — local and systemic delivery.Author: Yunching Chen, Dong Yu Gao, Leaf Huang
https://www.sciencedirect.com/science/article/pii/S0169409X14001033
In vivo miRNA delivery strategies for cancer therapy. A lot of work has been done in exploiting and evaluating the features of tumor and tumor microenvironment to improve RNA delivery. There are two classes of strategies for miRNA delivery — local and systemic delivery. 7.1. Local delivery of miRNAsAuthor: Yunching Chen, Dong Yu Gao, Leaf Huang
https://www.hindawi.com/journals/ije/2019/6782653/
Compared with commercially available magnetic vectors (Magnetofectamine, CombiMag), the miR/PEI/MNP complex formulations are not inferior with respect to miRNA uptake rates and cytotoxic effects, implying their great potential for in vivo therapeutic miRNA delivery to treat degenerative bone diseases as a result of MSC dysfunction .Author: Xi Sun, Qi Guo, Wenhua Wei, Stephen Robertson, Ying Yuan, Xianghang Luo
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995415/
May 05, 2010 · In vivo quantification of Ago2-associated siRNA and target gene silencing. To further validate our method and gain quantitative insights into siRNA delivery in vivo, we performed a mouse study measuring both gene silencing and Ago2-associated siRNA strand levels from tissue samples.Author: Yi Pei, Paula J. Hancock, Hangchun Zhang, René Bartz, Craig Cherrin, Nathalie Innocent, Colin J. Pom...
https://pubs.acs.org/doi/10.1021/acsnano.9b03343
A major challenge in myocardial infarction (MI)-related heart failure treatment using microRNA is the efficient and sustainable delivery of miRNAs into myocardium to achieve functional improvement through stimulation of intrinsic myocardial restoration. In this study, we established an in vivo delivery system using polymeric nanoparticles to carry miRNA (miNPs) for localized delivery within a ...Author: Huaxiao Yang, Xulei Qin, Huiyuan Wang, Xin Zhao, Yonggang Liu, Hung-Ta Wo, Chun Liu, Masataka Nishig...
https://www.sciencedirect.com/science/article/abs/pii/S0169409X14001033
However, one of the major challenges of miRNA-based cancer therapy is to achieve specific, efficient and safe systemic delivery of therapeutic miRNAs in vivo. This review discusses the key challenges to the development of the carriers for miRNA-based therapy and explores current strategies to systemically deliver miRNAs to cancer without ...Author: Yunching Chen, Dong Yu Gao, Leaf Huang
http://altogenlabs.com/rna-interference-rnai-services/in-vivo-sirna-delivery-and-tissue-targeting/
In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) In Vivo siRNA Delivery and Tissue Targeting. RNAi-induced gene silencing is used to study gene function in cultured cells (in vitro).Most often, these effects are induced by the introduction of small-interfering RNA (siRNA) or microRNA (miRNA).
https://www.nature.com/articles/s41551-017-0157-y
Nov 27, 2017 · An injectable hyaluronic acid hydrogel for the sustained delivery of miR-302 mimics to the heart promotes cardiomyocyte proliferation and improves cardiac function in …Author: Leo L. Wang, Ying Liu, Jennifer J. Chung, Tao Wang, Ann C. Gaffey, Minmin Lu, Christina A. Cavanaugh...
https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rnai-sirna/tech-notes/getting-started-with-rnai-in-vivo.html
The pMIR-REPORT Luciferase miRNA Expression Reporter Vector (as is, or with cloned targets) can be used in vivo and delivered by hydrodynamic injection to the mouse liver. siRNA can cause specific and potent knockdown of gene expression from a co-delivered reporter vector in liver cells.
https://www.medscape.com/viewarticle/837884_6
Nov 06, 2019 · In Vivo Delivery of Anti-miRNA . Both anti-miRNA and antisense RNA function through the formation of sense-antisense strands, although the two provide contrasting results.
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