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https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-support/gene-delivery-selection-guide.html
Researchers have a wide variety of gene delivery techniques to introduce plasmid DNA, siRNA or duplex RNAi, oligonucleotides, and RNA into eukaryotic cells for a variety of research and drug discovery applications. Lipid delivery using cationic lipids.
https://www.sciencedirect.com/science/article/abs/pii/S0889858805700046
There are two general categories of gene delivery systems, or vectors as they are commonly called: viral and nonviral vectors. Of the 210 gene therapy protocols that have been approved for phase I clinical trials, approximately 80% utilize virus vectors.37 …Author: Nelson A. Wivel, James M. Wilson
https://link.springer.com/chapter/10.1007/978-88-470-1643-9_3
Abstract. The success of any gene transfer procedure, either through in vivo inoculation of the genetic material or after gene transfer into the patient’s cells ex vivo, strictly depends upon the efficiency of nucleic acid internalization by the target cells.As a matter of fact, making gene transfer more efficient continues to represent the most relevant challenge to the clinical success of ...Author: Mauro Giacca
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417261/
Mar 12, 2019 · The non-viral gene delivery systems have also treated for the physicochemical approaches such as physical method and chemical method. Several kinds of gene delivery systems have discussed on the gene delivery systems of cationic biomedical polymers such as polysaccharides, polyethylenimine (PEI), and poly(L-lysine)(PLL) derivatives.Author: YK Sung, SW Kim
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Physical methods of nonviral gene delivery. Physical methods applied for in vitro and in vivo gene delivery are based on making transient penetration in cell membrane by mechanical, electrical, ultrasonic, hydrodynamic, or laser-based energy so that DNA entrance into the targeted cells is facilitated.
http://www.eurekaselect.com/106191/article
Gene delivery has attracted increasing interest as a highly promising therapeutic method to treat various diseases, including both genetic and acquired disorders. Viral-vectors based gene delivery can achieve higher transduction efficiency and long-term gene expression, …
http://www.genetherapynet.com/types-of-gene-therapy.html
Types of Gene Therapy. Virtually all cells in the human body contain genes, making them potential targets for gene therapy. However, these cells can be divided into two major categories: somatic cells (most cells of the body) or cells of the germline (eggs or sperm). In theory it is possible to transform either somatic cells or germ cells.
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