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https://www.sciencedirect.com/science/article/pii/S0889858805700046
Direct DNA Delivery. Of the major methods of nonviral, mediated gene transfer, direct DNA delivery is probably the simplest, because it involves injection of a plasmid DNA containing a transgene into an organ site of choice.Author: Nelson A. Wivel, James M. Wilson
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Physical methods of nonviral gene delivery. Physical methods applied for in vitro and in vivo gene delivery are based on making transient penetration in cell membrane by mechanical, electrical, ultrasonic, hydrodynamic, or laser-based energy so that DNA entrance into the targeted cells is facilitated.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722627/
Alternative gene therapy . Another means of using bacteria for gene therapy is the so-called Alternative gene therapy (AGT) approach, which is also known as bacterial protein delivery. It is based on the transfer of bacterially expressed therapeutic proteins to the host organism using genetically modified (transformed) bacteria.
https://en.wikipedia.org/wiki/Vectors_in_gene_therapy
Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
https://seekingalpha.com/article/4305556-gene-therapy-series-2-delivery-systems-in-gene-therapy-including-viral-vectors
Nov 11, 2019 · Continuing with the series of articles on gene therapy, this article will discuss the various delivery methods for gene therapies with a focus on viral vectors.The article also discusses the classific
https://www.slideshare.net/Aparajitha_Anne/gene-delivery-system
May 19, 2012 · Conclusion:• Gene therapy utilizes the delivery of DNA into cells, which can be done by several ways by a number of methods that is some of them are by viral methods and some are by the non-viral methods few are by the Hybrid methods.•
https://www.intechopen.com/books/recent-advances-in-novel-drug-carrier-systems/gene-delivery-systems-recent-progress-in-viral-and-non-viral-therapy
Conversely, in acquired diseases, a single gene cannot be defined as the sole cause of a disease. Gene therapy has increased in prominence, and has shown great potential in treating acquired and genetic diseases (Conwell and Huang, 2005). Gene delivery systems are categorized as: viral-based, non-viral-based and combined hybrid systems.Author: Erdal Cevher, Ali Demir Sezer, Emre Şefik Çağlar
https://www.sciencedirect.com/science/article/abs/pii/S0889858805700046
METHODS OF GENE DELIVERY. Author links open overlay panel Nelson A. Wivel MD ... the concept of human gene therapy has moved from a theoretic axis to the actual transfer of genes into the somatic cells of patients. 20 The concept itself is rather straightforward and involves the insertion of genes into cells for the expressed intent of ...Author: Nelson A. Wivel, James M. Wilson
https://www.ukessays.com/essays/biology/concepts-gene-therapy-2892.php
In vivo gene therapy: In this method the therapeutic gene is directly introduced into the body by injection or by inhalation with the help of a suitably designed vector. Gene Delivery Vectors: Key to Success in Gene therapy. Gene therapy, as a novel therapeutic modality, holds enormous promise for the treatment of a multitude of human diseases.
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