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https://www.cell.com/molecular-therapy-family/molecular-therapy/pdf/S1525-0016(16)31031-0.pdf
hours of siRNA treatment, CD11b+ macrophages and microglia cells were immunomagnetically isolated from the spleen and brain, respectively. To confirm delivery, small RNA fraction isolated from CD11b selected cells from the spleen and brain were tested for the presence of CyPB siRNA by quantitative reverse transcrip-
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2890121/
May 03, 2010 · RVG-9dR allows siRNA delivery to AchR-expressing macrophage and microglial cell lines in vitro. (a) Raw 264.7 and N9 cell lines were tested for AchR α7 subunit expression before and after activation with LPS by flow cytometry. Blue, no LPS treatment; Red, after LPS treatment (upper panel). The cells were tested for uptake of a FITC-labeled ...Author: Sang-Soo Kim, Chunting Ye, Priti Kumar, Isaac Chiu, Sandesh Subramanya, Haoquan Wu, Premlata Shankar...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839649/
Nov 06, 2019 · Delivery of nucleic acids into solid tumor environments remains a pressing challenge. This study examines the ability of macrophages to horizontally transfer small interfering RNA (siRNA) lipoplexes to cancer cells. Macrophages are a natural candidate …Author: Elizabeth C. Wayne, Christian Long, Matthew J. Haney, Elena V. Batrakova, Tina M. Leisner, Leslie V....
https://www.sciencedirect.com/science/article/pii/S0165242714000385
The manipulation of the RNA interference pathway using small interfering RNA (siRNA) has become the most frequently used gene silencing method. However, siRNA delivery into primary cells, especially primary macrophages, is often considered challenging.Author: Kirsty Jensen, Jennifer A. Anderson, Elizabeth J. Glass
https://www.sciencedirect.com/science/article/pii/S092849311930356X
Non-toxic, cationic curdlan nanoparticles for macrophage targeted gene delivery. • Higher uptake of the nanoparticles in macrophages than fibroblasts. • Ability to activate M1 phenotype macrophages to aid tumor regression. • Successful siRNA delivery to macrophages, with efficiency comparable to that of a transfection reagent. •Author: Rubaiya Yunus Basha, Geetha Venkatachalam, T.S. Sampath Kumar, Mukesh Doble
https://www.nature.com/articles/s41598-017-13032-9
Oct 10, 2017 · Delivery of siRNA against RELA by iRGD nanoparticles resulted in significant suppression of NF-κB1 expression, when compared to delivery of GFP siRNA using the same nanoparticles (Supplementary ...Author: Yin Ren, Jessica E. Sagers, Jessica E. Sagers, Lukas D. Landegger, Sangeeta N. Bhatia, Konstantina M...
https://pubs.acs.org/doi/10.1021/acsami.8b02073
Apr 12, 2018 · The CMI nanoparticles successfully delivered a dye-labeled siRNA to mouse peritoneal macrophages. The delivery efficiency was blocked by mannan, a natural ligand for a macrophage surface mannose receptor (CD206), but not by zymosan, a ligand for the dectin-1 receptor, which is also present on the surface of macrophages.Author: Tsogzolmaa Ganbold, Huricha Baigude
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