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https://pubs.acs.org/doi/10.1021/bc050226x
Oct 20, 2005 · Structural evolution of the carrier lipid/DNA complexes upon interaction with cellular lipids is hence suggested as a controlling factor in lipid-mediated DNA delivery. A strategy for optimizing lipofection is deduced. The behavior of a highly effective lipoplex formulation, DOTAP/DOPE, is found to conform to this “efficiency formula”.Author: Rumiana Koynova, Li Wang, Yury Tarahovsky, Robert C. MacDonald
https://www.researchgate.net/publication/7481655_Lipid_Phase_Control_of_DNA_Delivery
Lipid Phase Control of DNA Delivery. ... Lipid phase behavior is known to depend strongly on the chain molecular structure and the above relationships thus substantiate a view that cationic PC ...
https://pubs.acs.org/doi/full/10.1021/bc050226x
Formulations that are particularly effective DNA carriers form phases of highest negative interfacial curvature when mixed with anionic lipids. Structural evolution of lipoplexes upon interaction with cellular lipids is hence suggested as the controlling factor in lipid-mediated DNA delivery.Author: Rumiana Koynova, Li Wang, Yury Tarahovsky, Robert C. MacDonald
https://www.ncbi.nlm.nih.gov/pubmed/16287225
Lipid phase control of DNA delivery. Koynova R(1), Wang L, Tarahovsky Y, MacDonald RC. ... Structural evolution of the carrier lipid/DNA complexes upon interaction with cellular lipids is hence suggested as a controlling factor in lipid-mediated DNA delivery. A strategy for optimizing lipofection is deduced.Author: Rumiana Koynova, Li Wang, Yury Tarahovsky, Robert C. MacDonald
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2849731/
Apr 15, 2010 · Here we report a comparative investigation of cationic lipid-mediated delivery of RNA versus DNA vectors encoding the reporter gene green fluorescent protein (GFP) in Chinese Hamster Ovary (CHO) and NIH3T3 cells following chemical inhibition of proliferation, and in primary mixed neuronal cell cultures.Author: S. Zou, K. Scarfo, M.H. Nantz, J.G. Hecker
https://science.sciencemag.org/content/281/5373/78.full
Jul 03, 1998 · A two-dimensional columnar phase in mixtures of DNA complexed with cationic liposomes has been found in the lipid composition regime known to be significantly more efficient at transfecting mammalian cells in culture compared to the lamellar (Lα C) structure of cationic liposome–DNA complexes. The structure, derived from synchrotron x-ray diffraction, consists of DNA coated by …Author: Ilya Koltover, Tim Salditt, Joachim O. Rädler, Cyrus R. Safinya
https://www.osti.gov/biblio/1007592-natural-lipid-extracts-biomembrane-mimicking-lipid-compositions-disposed-form-nonlamellar-phases-release-dna-from-lipoplexes-most-efficiently
The transfection activity and the phase behavior of two novel cationic O-alkyl-phosphatidylcholines, 1,2-dioleoyl-sn-glycero-3-hexylphosphocholine (C6-DOPC) and 1,2-dierucoyl-sn-glycero-3-ethylphosphocholine (di22:1-EPC), have been examined with the aim of more completely understanding the mechanism of lipid-mediated DNA delivery.
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